DNA Extraction from Vegetative Tissue for Next-Generation Sequencing
DNA Extraction from Vegetative Tissue for Next-Generation Sequencing
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DOI：
10.1007/978-1-62703-715-0_1
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发表时间：
2014-01-01
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影响因子：
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通讯作者：
Furtado, Agnelo
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文献类型：
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作者：
Furtado, Agnelo
提取的DNA的质量对于分子生物学的几个应用是至关重要的。如果DNA要用于下一代测序(NGS)，则需要微克量的高质量DNA。此外，DNA必须基本上是高分子量的，才能用于文库准备和NGS测序。通过试剂盒过滤，可以很容易地去除分离DNA中污染的苯酚或淀粉。在这一章中，我们描述了一种简单的双试剂DNA提取方法，该方法提取的DNA质量和数量都很高，可用于包括NGS在内的不同应用。
The quality of extracted DNA is crucial for several applications in molecular biology. If the DNA is to be used for next-generation sequencing (NGS), then microgram quantities of good-quality DNA is required. In addition, the DNA must substantially be of high molecular weight so that it can be used for library preparation and NGS sequencing. Contaminating phenol or starch in the isolated DNA can be easily removed by filtration through kit-based cartridges. In this chapter we describe a simple two-reagent DNA extraction protocol which yields a high quality and quantity of DNA which can be used for different applications including NGS.