Dual function of coronatine as a bacterial virulence factor against plants: possible COI1-JAZ-independent role
Dual function of coronatine as a bacterial virulence factor against plants: possible COI1-JAZ-independent role
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DOI：
10.1039/c5ra20676f
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发表时间：
2016-01-01
期刊：
影响因子：
3.9
通讯作者：
Ueda, Minoru
中科院分区：
文献类型：
--
作者：
Egoshi, Syusuke;Takaoka, Yousuke;Ueda, Minoru
冠菌素（COR，1）是一种植物毒素，是植物激素（+）-7-异茉莉酰基-异亮氨酸（2）的结构模拟物。COR（1）作为COI1-JAZ共受体的配体发挥作用，COI1-JAZ共受体是2的唯一受体。近年来，1作为一种新的植物毒力因子引起了植物科学家的关注。细菌通过气孔侵入植物质外体。然后，宿主植物通过关闭气孔（气孔防御）来响应细菌入侵。COR（1）作为细菌化学武器，通过重新打开关闭的气孔来确保感染路径。该机制被认为涉及通过COI1-JAZ途径抑制脱落酸信号传导。因此，1通过消除植物免疫应答在植物-微生物相互作用中起重要作用。在这项研究中，我们合成了7个类似物1与天然存在的a-氨基酸，并评估其对气孔的影响，在一个模式植物，拟南芥。类似物的结构活性关系的研究加上遗传研究和计算机对接分析与COI1-JAZ强烈建议，气孔重开1诱导可能不依赖于COI1-JAZ信号通路。我们的研究结果表明，气孔重新开放触发1与传统的COI1-JAZ的行动模式。
Coronatine (COR, 1) is a phytotoxin and structural mimic of the plant hormone (+)-7-iso-jasmonoyl-Lisoleucine (2). COR (1) functions as a ligand of the COI1-JAZ co-receptor, which is the exclusive receptor of 2. Recently, a new role for 1 as a plant virulence factor for Pseudomonas syringae has attracted the attention of plant scientists. Bacteria invade the plant apoplast through stomatal pores. The host plant then responds to the bacterial invasion by closing the stomatal pores (stomatal defense). COR (1) functions as a bacterial chemical weapon that secures the path of infection by reopening the closed stomata. The mechanism is thought to involve inhibition of abscisic acid-signaling through the COI1-JAZ pathway. Thus, 1 plays an important role in plant-microbe interactions by abrogating the plant immune response. In this study, we synthesized seven analogues of 1 with naturally occurring a-amino acids and assessed their effect on stomata in a model plant, Arabidopsis thaliana. Structure-activity relationship studies of the analogues coupled with genetic studies and in silico docking analyses with COI1-JAZ strongly suggested that stomatal reopening induced by 1 may not rely on the COI1-JAZ signaling pathway. Our results suggest that stomatal reopening is triggered by 1 in conjunction with the conventional COI1-JAZ mode of action.