Borrelia miyamotoi Infections among Wild Rodents Show Age and Month Independence and Correlation with Ixodes persulcatus Larval Attachment in Hokkaido, Japan
Borrelia miyamotoi Infections among Wild Rodents Show Age and Month Independence and Correlation with Ixodes persulcatus Larval Attachment in Hokkaido, Japan
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DOI：
10.1089/vbz.2012.1027
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发表时间：
2013-02-01
影响因子：
2.1
通讯作者：
Tsubota, Toshio
中科院分区：
文献类型：
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作者：
Taylor, Kyle R.;Takano, Ai;Tsubota, Toshio
为了阐明宫本疏螺旋体是如何在北海道的环境中维持的，我们通过检测啮齿动物膀胱和血液样本以及整个蜱中的疏螺旋体flaB基因的一部分来检查全沟硬蜱在野生啮齿动物及其蜱媒中的流行情况。我们比较了B。Miyamotoi的感染率与Garinii疏螺旋体和Afzelii疏螺旋体的感染率相当，这两种疏螺旋体是也由野生啮齿动物携带的人类莱姆病病原体，并且由相同的媒介蜱传播。而B. garinii和B.在膀胱标本中，阿氏疟原虫在野生啮齿动物中的感染率显示出年龄依赖性（成年鼠分别为18.4%和9.9%，亚成年鼠分别为6.0%和3.4%），B.无论是血液样本（成人为4.2%，亚成人为7.9%）还是膀胱样本（成人为1.0%，亚成人为1.7%），Miyamotoi感染率均与年龄无关。此外，虽然B. garinii和B.阿氏杆菌感染率在月份间呈上升趋势（6月、7月[p < 0.05]和8月[p < 0.01]），成年啮齿类动物感染B的比率高于5月。7月和8月成鼠携带B的比例显著高于5月[p < 0.01]。afzelii），B. Miyamotoi感染率没有表现出明显的月份依赖性。这些月龄依赖性的差异使我们怀疑B。miyamotoi在野生啮齿类动物中可能不会发生持续感染，如B。garinii和B. Afzelii被认为是。此外，我们检查了啮齿动物暴露于I的程度。persulcatus蜱和幼虫在大部分蜱的活跃季节（5月至9月），并确定B。亚成体鼠的感染率与幼虫负荷相关（p < 0.01），提示幼虫在B的传播中可能起重要作用。对野生啮齿动物的影响。
To clarify how Borrelia miyamotoi is maintained in the environment in Hokkaido, we examined Ixodes persulcatus for its prevalence among wild rodents and its tick vector by detecting a portion of the borrelial flaB gene in rodent urinary bladder and blood samples, and from whole ticks. We compared B. miyamotoi infection rates to Borrelia garinii and Borrelia afzelii, which are human Lyme disease pathogens also carried by wild rodents, and which are transmitted by the same vector tick. Whereas B. garinii and B. afzelii showed age dependence of infection rates among wild rodents (18.4% and 9.9% among adults and 6.0% and 3.4% among sub-adults, respectively) when looking at urinary bladder samples, B. miyamotoi infection rates were not age dependent for either blood (4.2% among adults, and 7.9% among sub-adults) or urinary bladder samples (1.0% among adults, and 1.7% among sub-adults). Moreover, while B. garinii and B. afzelii infection rates showed increases across months (June, July [p < 0.05] and August [p < 0.01] had higher rates than in May for adult rodents with B. garinii, and July and August had higher rates than in May [p < 0.01] for adult rodents with B. afzelii), B. miyamotoi infection rates did not show significant month dependence. These differences in month and age dependence led us to suspect that B. miyamotoi may not develop persistent infections in wild rodents, as B. garinii and B. afzelii are thought to. Furthermore, we examined the extent of rodent exposure to I. persulcatus nymphs and larvae throughout most of the tick's active season (May through September), and determined that B. miyamotoi infection rates in sub-adult rodents were correlated with larval burden (p < 0.01), suggesting that larvae may be very important in transmission of B. miyamotoi to wild rodents.