Intestinal Absorption of Fibrinolytic and Proteolytic Lumbrokinase Extracted from Earthworm, Eisenia andrei
Intestinal Absorption of Fibrinolytic and Proteolytic Lumbrokinase Extracted from Earthworm, Eisenia andrei
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DOI：
10.4196/kjpp.2010.14.2.71
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发表时间：
2010-04-01
影响因子：
2
通讯作者：
Sohn, Uy Dong
中科院分区：
文献类型：
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作者：
Yan, Xiang Mei;Kim, Chung-Hyo;Sohn, Uy Dong
采用大鼠外翻肠囊法和在体闭环循环法研究了从安氏蚓中提取的纤溶和蛋白水解蚓激酶的肠吸收。从安得氏蚓中提取蚓激酶，制备了蚓激酶多克隆抗体。用蚯蚓激酶孵育的大鼠翻转肠囊的浆膜侧的纤维蛋白溶解活性和蛋白水解活性表现出剂量和时间依赖性模式。采用大鼠外翻肠囊法，通过免疫印迹法获得的丝氨酸蛋白酶溶液的免疫学结果表明，在33.6和54.7 kDa之间的蚓激酶蛋白主要被肠上皮吸收。此外，通过原位再循环方法获得的血浆组分的MALDI-TOF质谱分析证实蚓激酶F1被吸收到血液中。这些结果支持蚓激酶经口给药可从粘膜腔吸收进入血液的观点。
To investigate the intestinal absorption of a fibrinolytic and proteolytic lumbrokinase extracted from Eisenia andrei, we used rat everted gut sacs and an in situ closed-loop recirculation method. We extracted lumbrokinase from Eisenia andrei, and then raised polyclonal antibody against lumbrokinase. Fibrinolytic activity and proteolytic activity in the serosal side of rat everted gut sacs incubated with lumbrokinase showed dose- and time-dependent patterns. Immunological results obtained by western blotting serosal side solution using rat everted gut sacs method showed that lumbrokinase proteins between 33.6 and 54.7 kDa are absorbed mostly by the intestinal epithelium. Furthermore, MALDI-TOF mass spectrometric analysis of plasma fractions obtained by in situ recirculation method confirmed that lumbrokinase F1 is absorbed into blood. These results support the notion that lumbrokinase can be absorbed from mucosal lumen into blood by oral administration.