Mash2 acts cell autonomously in mouse spongiotrophoblast development
Mash2 acts cell autonomously in mouse spongiotrophoblast development
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DOI：
10.1006/dbio.1997.8685
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发表时间：
1997-10-01
影响因子：
2.7
通讯作者：
Nagy, A
中科院分区：
文献类型：
--
作者：
Tanaka, M;Gertsenstein, M;Nagy, A
Mash2基因编码一个基本的螺旋-环-螺旋转录因子，是果蝇无毛鳞状突基因在哺乳动物中的同源物之一。它在移植后小鼠胚胎的二倍体滋养层细胞中强烈表达。Mash2的定向突变表明，功能丧失会导致中期胚胎死亡，这是由于胎盘衰竭与缺乏海绵状滋养细胞和迷路滋养层减少有关。为了进一步研究Mash2在滋养层细胞系发育中的作用，我们结合Mash2突变体和野生型胚胎进行了嵌合分析。我们已经解决了影响所有三层滋养层细胞的Mash2突变胚胎的表型是由细胞自主还是非自主缺陷引起的问题，以及Mash2是否在海绵状滋养层和迷路滋养层的发育中都是必需的。结果表明，在胎盘嵌合后10.5天和12.5天，Mash2突变细胞对海绵滋养层没有贡献，提示Mash2基因的产物在海绵滋养层发育过程中是自主需要的细胞。然而，Mash2似乎不是迷宫状滋养细胞或巨细胞发育所必需的，因为在所分析的嵌合胎盘的滋养层中，Mash2突变细胞的比例很高。因此，我们可以得出结论，Mash2的主要细胞自主功能似乎参与了外胎盘锥体中二倍体滋养层细胞的发育，形成了成熟的绒毛尿囊膜胎盘的海绵滋养层。(C)1997年学术出版社。
The Mash2 gene, which encodes a basic helix-loop-helix transcription factor, is one of the mammalian homologues of the Drosophila achaete-scute genes. It is strongly expressed in diploid trophoblast cells of the postimplantation mouse embryo. Targeted mutagenesis of Mash2 revealed that loss of function results in embryonic lethality at midgestation, due to placental failure associated with a lack of spongiotrophoblast and reduced labyrinthine trophoblast layers. For the further study of Mash2 function in development of the trophoblast cell lineage, we have performed chimeric analysis combining Mash2 mutant and wild-type embryos. We have addressed the question of whether the phenotype of the Mash2 mutant embryo, which affects all of the three trophoblast cell layers, is caused by a cell autonomous or non-autonomous defect and whether Mash2 is required in both spongiotrophoblast and labyrinthine trophoblast development. Our results showed no contribution of Mash2 mutant cells to the spongiotrophoblast layer in chimeric placentae at 10.5 and 12.5 days postcoitum, suggesting that the product of the Mash2 gene is required cell autonomously during the development of the spongiotrophoblast. However, it seems that Mash2 is not required for development of labyrinthine trophoblast or giant cells, since high contributions of Mash2 mutant cells were observed in those trophoblast cell layers in the chimeric placentae analyzed. We can therefore conclude that the primary and cell-autonomous function of Mash2 appears to be an involvement in the development of diploid trophoblast cells in the ectoplacental cone to form the spongiotrophoblast cell layer of the mature chorioallantoic placenta. (C) 1997 Academic Press.