Quenched emission of fluorescence by ligand functionalized gold nanoparticles
Quenched emission of fluorescence by ligand functionalized gold nanoparticles
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DOI：
10.1023/b:jofl.0000031821.74706.ea
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发表时间：
2004-07-01
影响因子：
2.7
通讯作者：
Pérez-Luna, VH
中科院分区：
文献类型：
--
作者：
Aslan, K;Pérez-Luna, VH
提出了一种基于配体功能化的金纳米颗粒的荧光检测方案。该转换方案是基于金属表面对位于其附近(<；5 nm)的荧光团所产生的荧光发射的强烈猝灭。生物素化的金纳米颗粒与荧光团标记的抗生物素结合后，荧光发射强度降低。随后标记的抗生物素与D-生物素竞争性解离，导致荧光发射强度增加。这些相互作用是通过特定的分子识别方式发生的，因为当抗生物素的结合部位在与金纳米颗粒相互作用之前与D-生物素饱和时，没有观察到荧光发射强度的变化。
A fluorescence-based detection scheme that uses ligand functionalized gold nanoparticles is proposed. The transduction scheme is based on the strong quenching of the fluorescence emission exerted by metallic surfaces on fluorophores positioned in their immediate vicinity (< 5 nm). Binding of fluorophore-labeled anti-biotin to biotinylated gold nanoparticles resulted in decreased fluorescence emission intensity. Subsequent competitive dissociation of labeled anti-biotin with D-biotin resulted in increased fluorescence emission intensity. These interactions occurred by means of specific molecular recognition because when the binding sites of anti-biotin were saturated with D-biotin prior to interaction with the gold nanoparticles; changes in the fluorescence emission intensity were not observed.