Differences in capacities of in vitro organ regeneration between two Arabidopsis ecotypes Wassilewskija and Columbia
Differences in capacities of in vitro organ regeneration between two Arabidopsis ecotypes Wassilewskija and Columbia
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DOI：
10.1007/s11240-012-0216-8
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发表时间：
2012
期刊：
影响因子：
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通讯作者：
X. Zhao;Y. Su;C. L. Zhang;Liang Wang;X. Li;X. Zhang
中科院分区：
文献类型：
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作者：
X. Zhao;Y. Su;C. L. Zhang;Liang Wang;X. Li;X. Zhang
体外器官发生受到很好的控制，因此为研究植物器官发育的机制提供了一个理想的系统。虽然外源激素在决定体外再生器官类型中的重要作用已经被研究很久了，但目前关于其他调节器官再生的关键因素的信息有限。在这里，我们报道了简单而有效的一步法来评估两种不同生态型拟南芥的花序茎体外器官发生能力。在含有216种外源生长素和细胞分裂素的培养基上，花序茎段外植体分化出不同类型的器官，包括茎和根。此外，我们还发现，在不同激素组合下，Wassilewski ja生态型的不定芽再生能力远高于哥伦比亚生态型，说明生态型是决定新器官发生的重要因素。我们的结果还表明，生长素和细胞分裂素生物合成相关基因的表达模式与两个生态型之间器官发生能力的差异有关。因此，体外器官发生可能是由生态型通过调节内源激素的生物合成来调节的。
In vitro organogenesis is well-controlled and thus provides an ideal system to study mechanisms of plant organ development. Although it has been well investigated for a long time that exogenous hormones play important roles in determining the types of organs regenerated in vitro, there is currently limited information available for other key factors that mediate de novo organ regeneration. Here, we reported simple and efficient one-step processes for evaluating capacities of inflorescence stem-derived in vitro organogenesis between two different ecotypes in Arabidopsis. Different types of organs, including shoots and roots were initiated from inflorescence stem explants cultured on the media containing 216 combinations of exogenous auxin and cytokinin. Further, we showed that Wassilewskija ecotype had the much higher shoot regeneration capacity than Columbia with different combinations of hormones, indicating that the ecotype is an essential factor determining de novo organogenesis. Our results also suggested that the defined expression patterns of genes involved in auxin and cytokinin biosynthesis were correlated with the variations in organogenesis capacities between the two ecotypes. Thus, in vitro organogenesis is likely regulated by ecotypes through mediating endogenous hormonal biosynthesis.