A High-Sucrose Isocaloric Pair-Fed Model Induces Obesity and Impairs NDUFB6 Gene Function in Rat Adipose Tissue
A High-Sucrose Isocaloric Pair-Fed Model Induces Obesity and Impairs NDUFB6 Gene Function in Rat Adipose Tissue
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DOI：
10.1159/000308465
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发表时间：
2009-01-01
影响因子：
--
通讯作者：
Martinez, J. A.
中科院分区：
文献类型：
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作者：
Lomba, A.;Milagro, F. I.;Martinez, J. A.
背景/目的：膳食中大量营养素含量的变化与能量代谢的改变和肥胖的发生有关。然而，关于高糖（HS）饮食的致肥胖效应存在争议，这通常会导致肥胖和胰岛素抵抗，但有时与体重增加减少有关。本研究的目的是调查的消费对喂饲HS饮食对大鼠的体重增加和能量稳态的影响，以及评估对脂肪组织中的NADH脱氢酶（泛醌）1 β亚复合物6（NDUFB 6）基因的表达的影响。方法/结果：虽然两个饮食组，即HS和控制，成对喂养（等能喂养），HS饮食增加肥胖和降低血浆总和高密度脂蛋白胆固醇水平。虽然在血清葡萄糖、胰岛素、脂联素、游离脂肪酸和肝脏丙二醛方面未发现显著差异，但观察到血清和肝脏甘油三酯轻微增加。有趣的是，NDUFB 6的基因表达，线粒体内膜蛋白参与线粒体电子传递，减少附睾脂肪组织相比，对照组。结论：这些结果表明，显然是第一次，高糖饮食似乎诱导脂肪组织中的线粒体功能障碍，这可能与体重增加和代谢障碍有关。版权所有（C）2010 S. Karger AG，巴塞尔
Background/Aim: Changes in dietary macronutrient content are involved in altered energy metabolism and obesity development. However, there are controversial views about the obesogenic effects of high-sucrose (HS) diets, which usually lead to obesity and insulin resistance but are sometimes associated with reduced weight gain. The aim of this study was to investigate the effect of consumption of a pair-fed HS diet on weight gain and energy homeostasis in rats, as well as to assess the effects on expression of the NADH dehydrogenase (ubiquinone) 1 beta subcomplex 6 (NDUFB6) gene in adipose tissue. Methods/Results: Although both dietary groups, i.e. HS and control, were pair-fed (isoenergetic feeding), the HS diet increased adiposity and decreased plasma total and high-density lipoprotein cholesterol levels. While no significant differences were found with regard to serum glucose, insulin, adiponectin, free fatty acids and liver malondialdehyde, a slight increase in serum and liver triglycerides was observed. Interestingly, the gene expression of NDUFB6, an inner mitochondrial membrane protein involved in mitochondrial electron transport, was reduced in epididymal adipose tissue when compared to the control-fed group. Conclusion: These results suggest, apparently for the first time, that high-sugar diets appear to induce mitochondrial dysfunction in adipose tissue, which may be related to greater weight gain and metabolic impairment. Copyright (C) 2010 S. Karger AG, Basel