Bacterial quorum sensing quenching activity of Lysobacter leucyl aminopeptidase acts by interacting with autoinducer synthase.
Bacterial quorum sensing quenching activity of Lysobacter leucyl aminopeptidase acts by interacting with autoinducer synthase.
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溶杆菌亮氨肽酶的细菌群体感应猝灭活性通过与自诱导剂合酶相互作用起作用
DOI：
10.1016/j.csbj.2021.11.017
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发表时间：
2021
影响因子：
6
通讯作者：
Qian G
中科院分区：
文献类型：
--
作者：
Liao J;Shen D;Lin L;Chen H;Jin Y;Chou SH;Yu XQ;Li T;Qian G
Schematic illustration of AHL quorum quenching strategies. Three known AHL quorum-quenching strategies (left). Quenching of AHL signaling by inhibiting AHL synthesis (middle gray arrow) or AHL-receptor binding (bottom gray arrow) by small molecular chemicals or enzymatically degrading AHL (top gray arrow) are indicated. In the right panel, we proposed a model of LqqP-mediated quenching of PcoI-dependent AHL signaling, representing a new quorum-quenching strategy. The direct binding of LqqP and PcoI reduces the abundance of PcoI (capable of AHL synthesis) in vivo through an unknown mechanism. LuxI and PcoI: AHL synthase, LuxR and PcoR: AHL receptor transcriptional regulator. Acyl-homoserine lactone (AHL) is the most studied autoinducer in gram-negative bacteria controlling infections of various pathogens. Quenching of AHL signaling by inhibiting AHL synthesis or AHL-receptor binding via small molecular chemicals or enzymatically degrading AHL is commonly used to block bacterial infections. Here, we describe a new quorum-quenching strategy that directly “acquires” bacterial genes/proteins through a defined platform. We artificially expressed a typical AHL synthase gene pcoI from the biocontrol Pseudomonas fluorescens 2P24 in the antifungal bacterium Lysobacter enzymogenes OH11 lacking AHL production. This step led to the discovery of multiple PcoI interacting protein candidates from L. enzymogenes. The individual expression of these candidate genes in 2P24 led to the identification of Le0959, which encodes leucyl aminopeptidase, an effective protein that inhibits AHL synthesis in 2P24. Therefore, we define Le0959 as LqqP (Lysobacterquorum-quenching protein). The expression of pcoI in E. coli could produce AHL, and the introduction of lqqP into E. coli expressing pcoI could prevent the production of AHL. LqqP directly binds to PcoI, and this protein–protein binding reduced the abundance of free PcoI (capable of AHL synthesis) in vivo, thereby blocking PcoI-dependent AHL production. Overall, this study highlights the discovery of LqqP in quenching AHL quorum sensing by binding to AHL synthase via developing a previously-uncharacterized screening technique for bacterial quorum quenching.
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影响因子：
3.2
作者：
BRINT, JM;OHMAN, DE
通讯作者：
OHMAN, DE
影响因子：
5
作者：
Han, Yong;Wang, Yan;Tombosa, Simon;Wright, Stephen;Huffman, Justin;Yuen, Gary;Qian, Guoliang;Liu, Fengquan;Shen, Yuemao;Du, Liangcheng
通讯作者：
Du, Liangcheng
影响因子：
3.6
作者：
Lin, YH;Xu, JL;Zhang, LH
通讯作者：
Zhang, LH
影响因子：
3.2
作者：
Hoang, TT;Schweizer, HP
通讯作者：
Schweizer, HP
DOI：
10.1038/s41576-021-00363-7
发表时间：
2021-09
期刊：
Nature reviews. Genetics
影响因子：
--
作者：
Medema MH;de Rond T;Moore BS
通讯作者：
Moore BS