Hypothetical mode of action of earthworm extract with hepatoprotective and antioxidant properties
Hypothetical mode of action of earthworm extract with hepatoprotective and antioxidant properties
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DOI：
10.1631/jzus.b0720194
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发表时间：
2008-02-01
影响因子：
5.1
通讯作者：
Cooper, Edwin L.
中科院分区：
文献类型：
--
作者：
Balamurugan, Mariappan;Parthasarathi, Kasi;Cooper, Edwin L.
以Wistar白化病大鼠为实验动物，研究了毛里求斯兰皮藤（Lampito mauritii，Kinberg）提取物（EE）对扑热息痛诱导的肝损伤的保护作用，并与标准保肝药水飞蓟素（silymarin）进行比较。我们观察到肝脏抗氧化剂，如谷胱甘肽（GSH），超氧化物歧化酶（SOD），谷胱甘肽过氧化物酶（GPx）和过氧化氢酶（CAT）和血清总蛋白减少，血清碱性磷酸酶（ALP），血清天冬氨酸氨基转移酶（AST），血清丙氨酸氨基转移酶（ALT），胆红素和肝脏硫代巴比妥酸反应物质（TBARS），由于对乙酰氨基酚给药大鼠（2 g/kg）的肝损伤。相反，不同剂量EE（100、200和300 mg/kg）可使大鼠肝脏GSH、SOD、GPx、CAT活性和血清总蛋白水平升高，血清ALP、AST、ALT、胆红素和肝脏TBARS含量降低，与保肝药水飞蓟素（150 mg/kg）相似。上述参数表明EE的作用方式可能表明EE一方面阻止活性氧基团的形成，或清除这些基团，从而防止对肝细胞的损伤，另一方面调节肝组织中负责合成抗氧化酶如GPx、CAT和SOD的基因，并降低血清酶活性如ALP，AST和ALT。
The hepatoprotective potential of earthworm extract (EE) (Lampito mauritii, Kinberg) was evaluated against paracetamol-induced liver injury in Wistar albino rat, in comparison with silymarin, the standard hepatoprotective drug. We observed a reduction in liver antioxidants, such as glutathione (GSH), superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) and in serum total protein, and an increase in serum alkaline phosphatase (ALP), serum aspertate aminotranferase (AST), serum alanine aminotranferase (ALT), bilirubin and liver thiobarbituric acid reactive substances (TBARS) due to liver injury in the paracetamol-administered rats (2g/kg). On the contrary, increased activities of liver GSH, SOD, GPx, CAT and serum total protein level, and decrease in the contents of serum ALP, AST, ALT, bilirubin and liver TBARS were observed in rats administered with different doses of EE (100, 200 and 300 mg/kg), which are similar to the activities of hepatoprotective drug silymarin (150 mg/kg). The mode of action of EE as evidenced by the above parameters may suggest that EE, on the one hand, prevents the formation of the reactive oxygen groups, or scavenges these groups, thereby preventing the damage on the hepatic cells, and, on the other hand, modulates the genes responsible for synthesis of antioxidant enzymes such as GPx, CAT and SOD in liver tissue and decreases the serum enzymatic activities such as ALP, AST and ALT.