The use of synthetic peptide combinatorial libraries for the identification of bioactive peptides.
The use of synthetic peptide combinatorial libraries for the identification of bioactive peptides.
复制标题
使用合成肽组合库来鉴定生物活性肽。
作者：
R. Houghten;J. Appel;S. Blondelle;J. Cuervo;C. Dooley;C. Pinilla
综述了合成肽组合文库（SPCLs）的系统制备，每个文库由数千万个肽组成，这些肽可以在现有的诊断或免疫相关的体外测定系统中进行筛选。最佳肽序列的鉴定是通过在溶液中筛选SPCL实现的，SPCL的每个元件由超过100，000个等摩尔表示的非支持物结合肽组成，沿着迭代合成和筛选过程。提供了实例，其中使用总共由52，128，400个乙酰化六肽组成的SPCL连同迭代选择过程沿着，以使用竞争性酶联免疫吸附测定精确地鉴定由单克隆抗体识别的肽的抗原决定簇。该相同的文库还用于在细菌生长抑制测定中开发高效的抗微生物肽。一个单独的非乙酰化的SPCL被用来筛选和识别高亲和力的肽配体，使用阿片类放射性受体结合测定。
The systematic preparation of synthetic peptide combinatorial libraries (SPCLs), each composed of tens of millions of peptides that can be screened in existing diagnostically or pharmacologically relevant in vitro assay systems, is reviewed. The identification of optimal peptide sequences has been achieved through the screening in solution of SPCLs, each element of which is composed of more than 100,000 nonsupport-bound peptides in equimolar representation, along with an iterative synthesis and screening process. Examples are presented in which an SPCL, composed in total of 52,128,400 acetylated hexa-peptides, is used along with an iterative selection process to precisely identify the antigenic determinant of a peptide recognized by a monoclonal antibody using competitive enzyme-linked immunosorbent assay. This same library was also used to develop highly potent antimicrobial peptides in bacterial growth inhibition assays. A separate non-acetylated SPCL was used to screen and identify high affinity peptide ligands using an opiate radio-receptor binding assay.