A miR-18a binding-site polymorphism in CDC42 3UTR affects CDC42 mRNA expression in placentas and is associated with litter size in pigs
A miR-18a binding-site polymorphism in CDC42 3UTR affects CDC42 mRNA expression in placentas and is associated with litter size in pigs
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CDC42 3-UTR 中的 miR-18a 结合位点多态性影响胎盘中 CDC42 mRNA 的表达，并与猪的产仔数相关
DOI：
10.1007/s00335-018-9788-x
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发表时间：
2019-02-01
期刊：
影响因子：
2.5
通讯作者：
Yu, Mei
中科院分区：
文献类型：
--
作者：
Liu, Ruize;Deng, Dadong;Yu, Mei
越来越多的证据表明，靶基因3-非翻译区（3UTR）的miRNA结合位点多态性可能影响该基因的表达，并可能与多种复杂性状相关。本研究发现，在猪胎盘发育的关键阶段，miR-18a和细胞分裂周期42 (CDC42) mRNA的表达呈负相关，在猪胎盘中存在差异表达。rs55618224 （T b> C）是CDC42 3UTR区域的一个SNP，与miR-18a种子完全互补，可以通过改变它们的结合亲和力来影响miR-18a对CDC42基因的相关调控。此外，CDC42 mRNA在TT纯合子胎盘中的表达水平高于CC纯合子胎盘。此外，我们还发现rs55618224与每窝产仔数之间存在显著关联。这些结果表明，CDC42 3UTR中miR-18a结合位点多态性可能通过调控CDC42基因影响猪胎盘产仔数。
Increasing evidence suggests that miRNA binding-site polymorphism in the 3-untranslated region (3UTR) of a target gene could affect that gene's expression, and can be associated with a variety of complex traits. In this study, we find that miR-18a and cell division cycle 42 (CDC42) mRNA, whose expression was inversely correlated, are differentially expressed in porcine placentas during critical stages of placental development. rs55618224 (T>C), a SNP in the 3UTR region of CDC42 that is perfectly complementary to the miR-18a seed could influence miR-18a-related regulation of CDC42 gene by altering their binding affinity. In addition, CDC42 mRNA was found to have higher expression level in the homozygous TT placentas as compared to those homozygous CC placentas in pigs. Furthermore, we identified a significant association between rs55618224 and total number born per litter. These results suggest the miR-18a binding-site polymorphism in CDC42 3UTR may impact litter size by regulation of CDC42 gene in porcine placentas.