Effect of single injection of recombinant human bone morphogenetic protein-2-loaded artificial collagen-like peptide in a mouse segmental bone transport model
Effect of single injection of recombinant human bone morphogenetic protein-2-loaded artificial collagen-like peptide in a mouse segmental bone transport model
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单次注射重组人骨形态发生蛋白2人工胶原样肽对小鼠节段骨运输模型的影响
DOI：
10.1155/2019/1014594
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发表时间：
2019
期刊：
影响因子：
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通讯作者：
Takaso M.
中科院分区：
文献类型：
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作者：
Tazawa R;Minehara H;Matsuura T;Kawamura T;Uchida K;Saito W;Inoue G;Takaso M.
本研究旨在研究在小鼠节段性骨运输（SBT）模型中，单次注射重组人骨形态发生蛋白-2-负载的人工胶原样肽凝胶（rhBMP-2/ACG）是否加速骨缺损部位的固结和对接部位的骨愈合。在小鼠股骨中产生临界尺寸的骨缺损（2 mm），随后使用具有外固定器的SBT进行重建。将小鼠分为四个治疗组：CONT组（不动对照组）、0.2组（骨段移动0.2 mm/天，持续10天）、1.0组（骨段移动1.0 mm/天，持续2天）和1.0/BMP-2组（将rhBMP-2/ACG注射到骨缺损中，骨段移动1.0 mm/天，持续2天）。在8周内通过影像学和组织学评价骨缺损部位的巩固和对接部位的骨愈合。第0.2组的骨体积和骨矿物质含量显著高于第1.0组。第0.2组显示骨缺损部位术后8周髓管重建的证据。然而，在组1.0中，骨缺损部位的再生骨成熟较差，近端和远端骨之间的中心区域主要由纤维和脂肪组织块组成。与组1.0相比，组1.0/BMP-2具有更高的骨体积和骨矿物质含量，并且所有小鼠在骨缺损和对接部位实现骨愈合。单次注射rhBMP-2/ACG联合SBT可有效促进大面积骨缺损的骨愈合。
This study aimed to investigate whether a single injection of recombinant human bone morphogenetic protein‐2‐loaded artificial collagen‐like peptide gel (rhBMP‐2/ACG) accelerates consolidation at the bone defect site and bone union at the docking site in a mouse segmental bone transport (SBT) model. A critical sized bone defect (2 mm) was created in the femur of mice and subsequently reconstructed using SBT with an external fixator. Mice were divided into four treatment groups: Group CONT (immobile control), Group 0.2 (bone segments moved 0.2 mm/day for 10 days), Group 1.0 (bone segments moved 1.0 mm/day for 2 days), and Group 1.0/BMP‐2 (rhBMP‐2/ACG injected into the bone defect and segments moved 1.0 mm/day for 2 days). Consolidation at the bone defect site and bone union at the docking site was evaluated radiologically and histologically across eight weeks. Bone volume and bone mineral content were significantly higher in Group 0.2 than in Group 1.0. Group 0.2 showed evidence of rebuilding of the medullary canal eight weeks after surgery at the bone defect site. However, in Group 1.0, maturation of regenerative bone at the bone defect site was poor, with the central area between the proximal and distal bone composed mainly of masses of fibrous and adipose tissue. Group 1.0/BMP‐2 had higher bone volume and bone mineral content compared to Group 1.0, and all mice achieved bone union at the bone defect and docking sites. Single injection of rhBMP‐2/ACG combined with SBT may be effective for enhancing bone healing in large bone defects.