Characterization of expression of matrix metalloproteinases and tissue inhibitors of metalloproteinases in prostate cancer cell lines
Characterization of expression of matrix metalloproteinases and tissue inhibitors of metalloproteinases in prostate cancer cell lines
复制标题
DOI：
10.1038/sj.pcan.4500609
复制
发表时间：
2003-01-01
影响因子：
4.8
通讯作者：
Russell, PJ
中科院分区：
文献类型：
--
作者：
Daja, MM;Niu, X;Russell, PJ
基质中某些基质金属蛋白酶（MMPs）的表达与前列腺癌中肿瘤负荷的增加有关。我们研究了MMPs和内源性抑制剂（金属蛋白酶组织抑制剂，TIMPs）的mRNA（通过RT-PCR）和蛋白质（通过酶谱法和蛋白质印迹法）在两个亲本上皮性前列腺癌细胞系和增加侵袭/转移潜能的亚系中的表达。膜型MMPs，MT 1-MMP和MT3-MMP mRNA的表达在PC 3衍生的细胞系中高于在LNCaP衍生的细胞系中，而MT 2-MMP mRNA的表达在LNCaP衍生的细胞系中高于在PC 3衍生的细胞系中。活性MT 1、MT 2和MT3-MMP蛋白水平在所有细胞系中相似，但在更具侵袭性的亚细胞系中，指示潜在MMP活化的加工MT-MMP增加。MMP-1、MMP-13和TIMP-1的表达在侵袭性更强的亚系中更高，并且可能与侵袭/转移能力有关。MMP-1和MMP-13表达的调节可能为治疗前列腺癌患者提供重要的治疗选择。
Stromal expression of some matrix metalloproteinases (MMPs) has been associated with increasing tumour burden in prostate cancer. We investigated the expression of mRNA (by RT-PCR) and protein (by zymography and western blotting) of MMPs and endogenous inhibitors (tissue inhibitors of metalloproteinases, TIMPs) in two parent epithelial prostate cancer cell lines and sublines of increasing invasive/metastatic potential. Expression of membrane type MMPs, MT1-MMP and MT3-MMP mRNA was higher in PC3-derived than in LNCaP-derived lines, whereas MT2-MMP mRNA expression was higher in the LNCaP derived than in PC3-derived cell lines. Active MT1, MT2 and MT3-MMP protein levels were similar in all lines, but processed MT-MMPs, indicative of latent MMP activation, were increased in more aggressive sublines. Expression of MMP-1, MMP-13 and TIMP-1 was higher in the more aggressive sublines and may be implicated in invasive/metastatic ability. Regulation of MMP-1 and MMP-13 expression may offer important therapeutic options for treating patients with prostate cancer.