Micromolding of shape-controlled, harvestable cell-laden hydrogels
Micromolding of shape-controlled, harvestable cell-laden hydrogels
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DOI：
10.1016/j.biomaterials.2006.06.005
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发表时间：
2006-11-01
期刊：
影响因子：
14
通讯作者：
Khademhosseini, Ali
中科院分区：
文献类型：
--
作者：
Yeh, Judy;Ling, Yibo;Khademhosseini, Ali
在水凝胶中封装哺乳动物细胞对于从组织工程到基于细胞的分析等各种应用具有很大的实用性。在这项工作中，我们提出了一种将活细胞封装在三维（3D）微尺度水凝胶（微凝胶）中的技术，这些水凝胶以可收获的独立单元的形式控制形状和大小。细胞悬浮在含有光引发剂的甲基丙烯酸透明质酸（MeHA）或聚乙二醇二丙烯酸酯（PEGDA）水凝胶前体溶液中，使用亲水聚二甲基硅氧烷（PDMS）压印进行微塑，并使用紫外线（UV）辐射交联。通过控制PDMS冲压件上的特征，控制成型水凝胶的尺寸和形状。微凝胶内的细胞分布均匀且保持活力。这些特定形状的微凝胶可以很容易地回收、培养和组装，以产生具有多种细胞类型空间分布控制的结构。该技术的进一步发展可能会导致应用于组织/器官再生和基于细胞的分析的3D共培养，其中模拟生理细胞-细胞相互作用的结构复杂性非常重要。(c) 2006 Elsevier Ltd.版权所有。
Encapsulation of mammalian cells within hydrogels has great utility for a variety of applications ranging from tissue engineering to cell-based assays. In this work, we present a technique to encapsulate live cells in three-dimensional (3D) microscale hydrogels (microgels) of controlled shapes and sizes in the form of harvestable free standing units. Cells were suspended in methacrylated hyaluronic acid (MeHA) or poly(ethylene glycol) diacrylate (PEGDA) hydrogel precursor solution containing photoinitiator, micromolded using a hydrophilic poly(dimethylsiloxane) (PDMS) stamp, and crosslinked using ultraviolet (UV) radiation. By controlling the features on the PDMS stamp, the size and shape of the molded hydrogels were controlled. Cells within microgels were well distributed and remained viable. These shape-specific microgels could be easily retrieved, cultured and potentially assembled to generate structures with controlled spatial distribution of multiple cell types. Further development of this technique may lead to applications in 3D co-cultures for tissue/organ regeneration and cell-based assays in which it is important to mimic the architectural intricacies of physiological cell-cell interactions. (c) 2006 Elsevier Ltd. All rights reserved.