Determination of pyronaridine in blood plasma by high-performance liquid chromatography for application in clinical pharmacological studies
Determination of pyronaridine in blood plasma by high-performance liquid chromatography for application in clinical pharmacological studies
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DOI：
10.1016/s0378-4347(96)00410-0
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发表时间：
1997-03-07
期刊：
影响因子：
--
通讯作者：
Navaratnam, V
中科院分区：
文献类型：
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作者：
Jayaraman, SD;Ismail, S;Navaratnam, V
建立了高效液相色谱-荧光检测法测定血浆中咯萘啶浓度的方法。该方法包括用磷酸盐缓冲液（pH 6.0，0.05 M）和乙醚-己烷（70：30%，v/v）进行液-液萃取，并在C-18柱（Nucleosil，250 × 4.6 mm I.D.，5 μ m粒径），乙腈-0.05 M磷酸盐缓冲液pH 6.0（60：40%，v/v）作为移动的流动相（1 ml/min），通过荧光检测（λ（ex）=267 nm，λ（em）=443 nm）。检测器响应线性范围为1000 ng，咯萘啶和奎宁的总回收率分别为90.0%和60.3%。该测定方法对血浆样品中10 ng/ml咯萘啶的测定具有足够的灵敏度，精密度可接受（
A method is described for the determination of pyronaridine in plasma using high-performance liquid chromatography with fluorescence detection. The method involves liquid-liquid extraction with phosphate buffer (pH 6.0, 0.05 M) and diethyl ether-hexane (70:30%, v/v) and chromatographic separation on a C-18 column (Nucleosil, 250X4.6 mm I.D., 5 mu m particle size) with acetonitrile-0.05 M phosphate buffer pH 6.0 (60:40%, v/v) as the mobile phase(1 ml/min) and detection by fluorescence (lambda(ex)=267 nm, lambda(em)=443 nm). The detector response is linear up to 1000 ng and the overall recoveries of pyronaridine and quinine were 90.0 and 60.3%, respectively. The assay procedure was adequately sensitive to measure 10 ng/ml pyronaridine in plasma samples with acceptable precision (