QUANTITATIVE IMAGING OF BORON, CALCIUM, MAGNESIUM, POTASSIUM, AND SODIUM DISTRIBUTIONS IN CULTURED-CELLS WITH ION MICROSCOPY
QUANTITATIVE IMAGING OF BORON, CALCIUM, MAGNESIUM, POTASSIUM, AND SODIUM DISTRIBUTIONS IN CULTURED-CELLS WITH ION MICROSCOPY
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DOI：
10.1021/ac00199a002
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发表时间：
1989-12-15
影响因子：
7.4
通讯作者：
MORRISON, GH
中科院分区：
文献类型：
--
作者：
AUSSERER, WA;LING, YC;MORRISON, GH
提出了一种将冻裂、冻干培养细胞的离子显微照片中的强度信息转换为局部干重元素浓度的方法。由培养细胞产生的匀浆用作校准标准品。 B、Ca、K、Mg 和 Na 相对于基质元素 C 的离子显微镜 (IM) 相对灵敏度因子由 IM 和细胞匀浆的电感耦合等离子体原子发射光谱分析的相关性确定。 IM 成像系统校准后，使用相对灵敏度因子确定培养的 Swiss 3T3 成纤维细胞中 B、Ca、K、Mg 和 Na 的局部细胞内浓度。细胞内 B 是通过细胞摄取 Na2B12H11SH 引入的，Na2B12H11SH 是硼中子捕获癌症治疗的候选治疗剂。 IM 细胞内浓度结果与已发表的电子探针 X 射线微量分析结果非常吻合。估计检测限在低至百万分之干重浓度范围内。
A method for the conversion of intensity information in ion micrographs of freeze-fractured, freeze-dried cultured cells to local dry weight elemental concentrations is presented. Homogenates generated from cultured cells are used as calibration standards. Ion microscope (IM) relative sensitivity factors for B, Ca, K, Mg, and Na with respect to the matrix element C are determined by the correlation of IM and inductively coupled plasma atomic emission spectrometry analyses of the cellular homogenates. After calibration of the IM imaging system, the relative sensitivity factors are used to determine local intracellular concentrations of B, Ca, K, Mg, and Na in cultured Swiss 3T3 fibroblasts. Intracellular B was introduced through cellular uptake of Na2B12H11SH, a candidate therapeutic agent for boron neutron capture cancer therapy. The IM intracellular concentration results show good agreement with published electron probe X-ray microanalysis results. Estimated detection limits are in the low- to subparts-per-million dry weight concentration range.