Edinburgh Research Explorer Development of a chicken 5 K microarray targeted towards immune function
Edinburgh Research Explorer Development of a chicken 5 K microarray targeted towards immune function
复制标题
爱丁堡研究探索者开发针对免疫功能的鸡 5 K 微阵列
DOI：
--
复制
发表时间：
--
期刊：
影响因子：
--
通讯作者：
S. Bollen
中科院分区：
文献类型：
--
作者：
S. Bollen
背景：鸡微阵列资源的开发是能够描述鸟类在不同挑战和刺激下发生的基因表达变化的重要步骤。免疫相关阵列的建立在确定与感染各种细菌和病毒疾病有关的宿主免疫反应方面具有很高的价值。结果：本文报道了鸡免疫相关基因文库的构建和包含5190个元件的微阵列的构建(一式两份)。该阵列上的克隆来自已知含有与免疫系统相关的高水平细胞的组织，即法氏囊、佩耶斯贴片、胸腺和脾。阵列上显示的是已知与现有鸡EST聚在一起的基因，以及我们的文库所独有的基因。其中一些
Background: The development of microarray resources for the chicken is an important step in being able to profile gene expression changes occurring in birds in response to different challenges and stimuli. The creation of an immune-related array is highly valuable in determining the host immune response in relation to infection with a wide variety of bacterial and viral diseases. Results: Here we report the development of chicken immune-related cDNA libraries and the subsequent construction of a microarray containing 5190 elements (in duplicate). Clones on the array originate from tissues known to contain high levels of cells related to the immune system, namely Bursa, Peyers patch, thymus and spleen. Represented on the array are genes that are known to cluster with existing chicken ESTs as well as genes that are unique to our libraries. Some of