Monitoring Gene Conversion in Budding Yeast by Southern Blot Analysis.
Monitoring Gene Conversion in Budding Yeast by Southern Blot Analysis.
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DOI：
10.1007/978-1-0716-0644-5_16
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发表时间：
2021
期刊：
影响因子：
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通讯作者：
Haber JE
中科院分区：
文献类型：
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作者：
Yamaguchi M;Haber JE
通过在芽殖酵母中使用可诱导的位点特异性双链断裂（DSB），可以真实的实时监测通过同源重组的断裂修复。描述了一种使用异位同源供体序列修复HO内切核酸酶诱导的DSB的方法。这些基因转换事件可以在有或没有交换的情况下发生，其产物被区分为不同大小的限制性内切酶片段。详细介绍了Southern杂交的方法。
By using an inducible site-specific double-strand break (DSB) in budding yeast, it is possible to monitor—in real time—the repair of the break by homologous recombination. A method is described using an ectopic homologous donor sequence to repair an HO endonuclease-induced DSB. These gene conversion events can occur with or without crossing-over, the products of which are distinguished as different-sized restriction endonuclease fragments. The method of Southern blotting is described in detail.