Evidence that an OX-2-positive cell can inhibit the stimulation of type 1 cytokine production by bone marrow-derived B7-1 (and B7-2)-positive dendritic cells.
Evidence that an OX-2-positive cell can inhibit the stimulation of type 1 cytokine production by bone marrow-derived B7-1 (and B7-2)-positive dendritic cells.
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DOI：
10.4049/jimmunol.162.2.774
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发表时间：
1999-01
影响因子：
4.4
通讯作者：
L. Gorczynski;Z. Chen;J. Hu;Y. Kai;J. Lei;V. Ramakrishna;R. Gorczynski
中科院分区：
文献类型：
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作者：
L. Gorczynski;Z. Chen;J. Hu;Y. Kai;J. Lei;V. Ramakrishna;R. Gorczynski
我们报道，当异体C57BL/6树突状细胞（DC）与C3H脾应答细胞孵卵时，肝单核非实质细胞（NPC）可以抑制免疫反应。从这些培养物中获得的细胞在C3H小鼠体内移植C57BL/6肾后存活率增加。我们还发现DC上OX-2表达的增加与细胞因子的产生和肾移植排斥反应的抑制有关。我们探讨了肝脏NPC的抑制是否与这些细胞的OX-2表达有关。将新鲜的C57BL/6脾源DC与C3H脾应答细胞和其他可能的协同调节细胞一起培养。后者来源于新鲜的C3H或C57BL/6肝NPC，或来源于经人Flt3配体静脉滴注10天的C3H或C57BL/6小鼠。不同的小鼠骨髓源DC群体也被用作假定的调节细胞来源，这些DC来自白细胞介素-4 +粒细胞-巨噬细胞- csf的骨髓培养。所有刺激培养的上清液检测不同细胞因子（IL-2、IL-4、ifn - γ和tgf - β）的功能表达。我们发现新鲜的C57BL/6脾DC诱导IL-2而不是IL-4的产生。来源的细胞显示抑制IL-2和ifn - γ的产生，促进IL-4和tgf - β的产生。通过半定量PCR和FACS分析发现，抑制作用与这些细胞上OX-2表达增加有关。通过大小分级，表达OX-2的细胞是NLDC145+细胞的一个亚群。我们的数据暗示了表达OX-2的细胞在常规异刺激DC诱导细胞因子产生的调节中的作用。
We reported that hepatic mononuclear, nonparenchymal cells (NPC) can inhibit the immune response seen when allogeneic C57BL/6 dendritic cells (DC) are incubated with C3H spleen responder cells. Cells derived from these cultures transfer increased survival of C57BL/6 renal allografts in C3H mice. We also found that increased expression of OX-2 on DC was associated with inhibition of cytokine production and renal allograft rejection. We explored whether inhibition by hepatic NPC was a function of OX-2 expression by these cells. Fresh C57BL/6 spleen-derived DC were cultured with C3H spleen responder cells and other putative coregulatory cells. The latter were derived from fresh C3H or C57BL/6 liver NPC, or from C3H or C57BL/6 mice treated for 10 days by i.v. infusion of human Flt3 ligand. Different populations of murine bone marrow-derived DC from cultures of bone marrow with IL-4 plus granulocyte-macrophage-CSF were also used as a source of putative regulator cells. Supernatants of all stimulated cultures were examined for functional expression of different cytokines (IL-2, IL-4, IFN-gamma, and TGFbeta). We found that fresh C57BL/6 splenic DC induced IL-2, not IL-4, production. Cells from the sources indicated inhibited IL-2 and IFN-gamma production and promoted IL-4 and TGFbeta production. Inhibition was associated with increased expression of OX-2 on these cells, as defined by semiquantitative PCR and FACS analysis. By size fractionation, cells expressing OX-2 were a subpopulation of NLDC145+ cells. Our data imply a role for cells expressing OX-2 in the regulation of induction of cytokine production by conventional allostimulatory DC.