Metabolic recovery from drowning by insect pupae
Metabolic recovery from drowning by insect pupae
复制标题
昆虫蛹溺水后的代谢恢复
DOI：
10.1242/jeb.144105
复制
发表时间：
2016
影响因子：
2.8
通讯作者：
Steven J. Lane
中科院分区：
文献类型：
--
作者：
H. Arthur Woods;Steven J. Lane
摘要许多陆地昆虫生活在间歇性泛滥的环境中，有些生命阶段可能在没有氧气的情况下在水下度过数天。我们测试的假设，陆生昆虫与地下pupelies显示呼吸适应生存缺氧，随后重建正常的呼吸模式。在实验中，将天南星的蛹浸入水中0至13天。 所有pupelies存活长达5天的浸泡，无论水是曝气或缺氧。 相比之下，五龄幼虫存活最多4小时的浸泡。 蛹期浸泡对成虫的大小和形态没有影响。浸泡后，puppatients最初发出大脉冲的二氧化碳。在随后的CO2排放低谷后，在浸泡1天的组中，气门活动恢复，然后平均CO2排放水平升高约1天，在3天和5天的浸泡处理中至少升高2天。 虽然CO2排放模式多种多样，但大多数植物都经历了一个以0.78-2.2 min为周期的循环模式排放CO2的时期。 Förster和Hetz（2010）以及Grieshaber和Terblanche（2015）的最新模型没有预测到这些高频周期，我们提出了几种可能的方法来使模型与我们的观测结果相一致。在浸泡过程中，腐殖酸积累乳酸，然后下降到低水平超过12-48小时。 浸泡3天和5天组的puestival在48小时后仍然具有升高的CO2排放率，这表明即使在乳酸恢复到低水平后，它们仍继续花费能量重建体内平衡。 尽管它们是陆生昆虫，但M. sexta可以承受长时间的浸泡和缺氧，随后可以重建体内平衡。尽管它们是陆地昆虫，但Manduca sexta的pupelium在水下存活了5天，并在恢复过程中表现出不同和不寻常的二氧化碳排放模式。
ABSTRACT Many terrestrial insects live in environments that flood intermittently, and some life stages may spend days underwater without access to oxygen. We tested the hypothesis that terrestrial insects with underground pupae show respiratory adaptations for surviving anoxia and subsequently reestablishing normal patterns of respiration. Pupae of Manduca sexta were experimentally immersed in water for between 0 and 13 days. All pupae survived up to 5 days of immersion regardless of whether the water was aerated or anoxic. By contrast, fifth-instar larvae survived a maximum of 4 h of immersion. There were no effects of immersion during the pupal period on adult size and morphology. After immersion, pupae initially emitted large pulses of CO2. After a subsequent trough in CO2 emission, spiracular activity resumed and average levels of CO2 emission were then elevated for approximately 1 day in the group immersed for 1 day and for at least 2 days in the 3- and 5-day immersion treatments. Although patterns of CO2 emission were diverse, most pupae went through a period during which they emitted CO2 in a cyclic pattern with periods of 0.78–2.2 min. These high-frequency cycles are not predicted by the recent models of Förster and Hetz (2010) and Grieshaber and Terblanche (2015), and we suggest several potential ways to reconcile the models with our observations. During immersion, pupae accumulated lactate, which then declined to low levels over 12–48 h. Pupae in the 3- and 5-day immersion groups still had elevated rates of CO2 emission after 48 h, suggesting that they continued to spend energy on reestablishing homeostasis even after lactate had returned to low levels. Despite their status as terrestrial insects, pupae of M. sexta can withstand long periods of immersion and anoxia and can reestablish homeostasis subsequently. Highlighted Article: Despite their status as terrestrial insects, pupae of Manduca sexta survived 5 days underwater and showed diverse and unusual patterns of CO2 emission during recovery.
影响因子：
2.2
作者：
Hahn, Daniel A.;Denlinger, David L.
通讯作者：
Denlinger, David L.