NUCLEOSOME-RESTRICTED ANTIBODIES ARE DETECTED BEFORE ANTI-DSDNA AND/OR ANTIHISTONE ANTIBODIES IN SERUM OF MRL-MP LPR/LPR AND +/+-MICE, AND ARE PRESENT IN KIDNEY ELUATES OF LUPUS MICE WITH PROTEINURIA
NUCLEOSOME-RESTRICTED ANTIBODIES ARE DETECTED BEFORE ANTI-DSDNA AND/OR ANTIHISTONE ANTIBODIES IN SERUM OF MRL-MP LPR/LPR AND +/+-MICE, AND ARE PRESENT IN KIDNEY ELUATES OF LUPUS MICE WITH PROTEINURIA
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DOI：
10.1002/art.1780371118
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发表时间：
1994-11-01
影响因子：
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通讯作者：
JACOB, L
中科院分区：
文献类型：
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作者：
AMOURA, Z;CHABRE, H;JACOB, L
目标。比较MRL小鼠对核小体的体液反应和对其单独成分(双链DNA[dsDNA]和组蛋白)的反应，并评估抗核小体抗体在肾脏免疫沉积中的作用。我们使用血清和肾脏洗脱液的酶联免疫吸附试验来检测针对纯化的核小体、dsDNA和组蛋白的抗体活性。抗核小体抗体出现在抗dsDNA和抗组蛋白抗体之前。部分抗核小体抗体仅与核小体反应，而不与其成分dsDNA和组蛋白反应。在蛋白尿MRL小鼠肾洗脱液中检测到这些核小体限制性抗体。我们的发现支持这样的观点，即核小体在抗核自身抗体的产生中起主要作用，并且抗核小体抗体可能参与了小鼠狼疮的肾炎形成过程。
Objective. To compare the humoral response to nucleosomes with the response to their individual components (double-stranded DNA [dsDNA] and histones) and to assess the involvement of antinucleosome antibodies in immune deposits in the kidney of MRL mice.Methods. We used enzyme-linked immunosorbent assays of sera and kidney eluates for antibody activity against purified nucleosomes, dsDNA, and histones.Results. Antinucleosome antibodies emerged before anti-dsDNA and antihistone antibodies. A fraction of antinucleosome antibodies reacted exclusively with nucleosomes and not with their components, dsDNA and histones. These nucleosome-restricted antibodies were detected in the proteinuric MRL mouse kidney eluate.Conclusion. Our findings support the notion that nucleosomes play a major role in the emergence of antinuclear autoantibodies and that antinucleosome antibodies might be involved in the nephritogenic process in murine lupus.