Detection of Localized Retinal Malfunction in Retinal Degeneration Model Using a Multielectrode Array System
Detection of Localized Retinal Malfunction in Retinal Degeneration Model Using a Multielectrode Array System
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DOI：
10.1002/jnr.22024
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发表时间：
2009-07-01
影响因子：
4.2
通讯作者：
Takahashi, Masayo
中科院分区：
文献类型：
--
作者：
Homma, Kohei;Osakada, Fumitaka;Takahashi, Masayo
光刺激通过光感受器中的光转导信号抑制视网膜暗电流。视网膜电图（ERG）检测暗电流的阻断作为ERG的a波。然而，标准的ERG代表视网膜的神经活动的总和，并且不能获得关于局部功能的信息。在这项研究中，我们使用一个多电极阵列（MEA）系统，并直接记录的焦点活动的光感受器的视网膜。从暗适应的啮齿动物中分离视网膜，并将其覆盖在电极阵列上，其中视网膜的玻璃体表面在电极阵列上。光刺激后，从每个电极记录负波。添加氨基丁酸，一种选择性激动剂的mGluR 6上表达的ON双极细胞，媒体没有阻止的反应的产生。反应的幅度随着视网膜发育的增加而增加。当视网膜局部受伤时，光引起的反应仅在视网膜的受伤区域减弱。还测试了从给予N-甲基-N-亚硝基脲（MNU）的大鼠中分离的视网膜。在MNU处理的大鼠的中央视网膜中，在注射MNU后反应进行性降低。相反，在周边视网膜，反应的幅度相对保留，与通过免疫组织化学观察到的视网膜厚度一致。总之，用MEA系统记录光诱发反应。MEA系统可用于检测光感受器的细微和局灶性激活。这种空间信息应该是有价值的，在调查当地的功能恢复治疗领域，如在基因转移或细胞移植。(C)2009 Wiley-Liss，Inc.
Light stimulation inhibits the retinal dark current through phototransduction signals in the photoreceptors. Electroretinography (ERG) detects the blockage of the dark current as the a-wave of the ERG. However, standard ERGs represent the summed neural activity of the retina, and information on localized functions cannot be obtained. In this study, we used a multielectrode array (MEA) system and directly recorded the focal activities of the photoreceptors of the retina. Retinas were isolated from dark-adapted rodents and were draped over the electrode array with vitreal surface of the retina on the electrode array. After light stimulation, negative waves were recorded from each electrode. Adding aminobutyric acid, a selective agonist of mGluR6 expressed on ON-bipolar cells, to the media did not block the generation of the responses. The amplitude of the response increased with increasing retinal development. When the retina was locally injured, light-elicited responses were diminished only in the injured areas of the retina. Retinas isolated from rats given N-methyl-N-nitrosourea (MNU) were also tested. In central retinas of MNU-treated rats, the responses were progressively decreased following injection of MNU. In contrast, in the peripheral retinas, amplitude of the responses was relatively retained, consistent with the retinal thickness observed by immunohistochemistry. In conclusion, light-evoked responses were recorded with the MEA system. The MEA system was useful for detecting subtle and focal activation of photoreceptors. This spatial information should be valuable in investigating local functional recovery in therapeutically treated areas, such as in gene transfer or cell transplantation. (C) 2009 Wiley-Liss, Inc.