A facile entrapment approach to construct PEGylated polyplexes for improving stability in physiological condition
A facile entrapment approach to construct PEGylated polyplexes for improving stability in physiological condition
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DOI：
10.1016/j.colsurfb.2007.03.008
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发表时间：
2007-08-01
影响因子：
5.8
通讯作者：
Shen, Jiacong
中科院分区：
文献类型：
--
作者：
Wang, Youxiang;Chen, Ping;Shen, Jiacong
聚乙二醇化多聚体已被证明可以提高DNA络合物的稳定性。但是，偶联反应可能会降低DNA的有效络合能力。在这里，我们描述了一种通过将聚乙二醇胆固醇醚(CPEG)包埋到聚合物中来构建聚乙二醇化聚合物的简单和有利的方法。有趣的是，CPEG的加入顺序对复合体在生理盐浓度下的稳定性有很大影响。在形成的PEI25k/DNA多聚体中加入CPEG对其稳定性没有影响。而在DNA溶液中加入CPEG和PEI25k混合物的“CPEG优先”法，在生理条件下，PEI25k/CPEG/DNA复合体表现出良好的抗聚集作用，并提高了转染率。这种不同的表现可能由CPEG被捕获的可能性来解释。利用CPEG中胆固醇基团与疏水电荷补偿核之间的疏水相互作用，采用“CPEG优先”法构建了聚乙二醇化聚合物。聚乙二醇包被显著提高了复合体在生理条件下的稳定性。这种简便的制备聚乙二醇化多聚体的方法在非病毒基因传递的研究和应用中具有很大的潜力。(C)2007 Elsevier B.V.保留所有权利。
PEGylated polyplexes had been proved to improve the stability of DNA complexes. However, the conjugation reaction might reduce the capacity of efficient DNA complexation. Herein we described an easy and favorable approach to construct PEGylated polyplexes via entrapping poly(ethylene glycol) cholesterol ether (CPEG) into polyplexes. It was of interest to find the addition sequence of CPEG had great effect on the stability of polyplexes in physiological salt concentration. The addition of CPEG into the formed PEI25k/DNA polyplexes had no effect to improve the stability. Whereas by the "CPEG first" method of adding CPEG and PEI25k mixture into the DNA solution, the PEI25k/CPEG/DNA polyplexes showed excellent anti-aggregation effect and enhanced transfection efficiency in physiological condition. The difference performance might be explained by the possibility of CPEG entrapment. By the "CPEG first" method, PEGylated polyplexes was constructed due to the hydrophobic interaction between the cholesterol group of CPEG and hydrophobic charged-compensated core. The PEG coating significantly improved the stability of polyplexes in physiological condition. This facile entrapment approach to prepare PEGylated polyplexes might have great potential in non-viral gene delivery research and application. (C) 2007 Elsevier B.V. All rights reserved.