The effect of pmpR on the type III secretion system in Pseudomonas aeruginosa
The effect of pmpR on the type III secretion system in Pseudomonas aeruginosa
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pmpR对铜绿假单胞菌Ⅲ型分泌系统的影响
DOI：
10.1007/s11434-011-4941-x
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发表时间：
2012-07-01
影响因子：
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通讯作者：
Duan KangMin
中科院分区：
文献类型：
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作者：
Liang HaiHua;Kong WeiNa;Duan KangMin
III型分泌系统(T3SS)在铜绿假单胞菌致病中起重要作用。在此之前，我们报道了一个未知蛋白PmpR可以通过直接与其启动子区域结合来调节群体感应系统中的一个重要调节因子PqsR。由于T3SS是由群体感应系统控制的，这里我们研究了PmpR和T3SS之间的关系。我们的数据显示，与野生型铜绿假单胞菌PAO1株相比，pmpR缺失突变株的T3SS基因exos、exoY、exoT和exsD的表达显著增加。DNA迁移率分析数据表明，PmpR间接影响T3SS。PmpR不太可能通过假单胞菌喹诺酮信号(PQS)控制T3SS，因为PQS负性调节T3SS，而PmpR负性调节PQS。PmpR对T3SS的影响似乎与PQ无关；需要进一步研究才能揭示潜在的调控途径。
The type III secretion system (T3SS) plays important roles in Pseudomonas aeruginosa pathogenicity. Previously, we reported that the uncharacterized protein PmpR could regulate pqsR, an important regulator in the quorum-sensing system, by directly binding to its promoter region. As the T3SS is controlled by the quorum-sensing system, here, we investigated the relationship between PmpR and the T3SS. Our data showed that expression of the T3SS genes exoS, exoY, exoT, and exsD was dramatically increased in a pmpR-deletion mutant compared with that in the wild-type P. aeruginosa strain PAO1. Data from DNA mobility assays indicated that PmpR affects the T3SS indirectly. It is unlikely that PmpR controls the T3SS via the Pseudomonas quinolone signal (PQS) because the PQS negatively regulates the T3SS, while pmpR negatively regulates the PQS. The effect of PmpR on the T3SS seems to be independent of the PQS; further investigation is required to uncover the underlying regulatory pathways.