E-SEM CT Observation of Collagen Scaffold Cultured Osteoblast Cells
E-SEM CT Observation of Collagen Scaffold Cultured Osteoblast Cells
复制标题
胶原支架培养成骨细胞的E-SEM CT观察
DOI：
10.1109/mhs.2016.7824240
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
T. Fukuda
中科院分区：
文献类型：
--
作者：
M. Nakajima;K. Nagao;M. Takeuchi;S. Yahata;K. Furusawa;T. Fukuda
在这项研究中，胶原蛋白支架的内部结构观察扫描电子显微镜-计算机断层扫描（SEM-CT）技术内的环境扫描电子显微镜（E-SEM）。E-SEM能够通过控制温度和压力来维持样品内部的水状态。通过对成骨细胞在一定培养时间内进行胶原支架的扫描电镜-CT观察，证实了成骨细胞的钙化变化。
In this study, the internal structure of collagen scaffold is observed by a scanning electron microscope-computed tomography (SEM-CT) technique operated inside an environmental-scanning electron microscope (E-SEM). The E-SEM is able to use maintain the aqueous condition inside the sample by controlling the temperature and pressure. We demonstrated the SEM-CT observation of collagen scaffold by culturing osteoblast cells in certain culture term, and confirmed the calcification change of osteoblast cells.