Layer-by-Layer Assembly of a Polysaccharide “Armor” on the Cell Surface Enabling the Prophylaxis of Virus Infection
Layer-by-Layer Assembly of a Polysaccharide “Armor” on the Cell Surface Enabling the Prophylaxis of Virus Infection
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DOI：
10.1021/acsami.2c03442
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发表时间：
2022-05
影响因子：
9.5
通讯作者：
Zhiqiang Nie;Y. Li;Xinxin Li;Youqian Xu;Guanyuan Yang;M. Ke;Xiaohang Qu;Yinhua Qin;Ju Tan;Yonghong Fan;Chuhong Zhu
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文献类型：
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作者：
Zhiqiang Nie;Y. Li;Xinxin Li;Youqian Xu;Guanyuan Yang;M. Ke;Xiaohang Qu;Yinhua Qin;Ju Tan;Yonghong Fan;Chuhong Zhu
空气传播的病原体，如世界传播的严重急性呼吸综合征冠状病毒2型（SARS-CoV-2），通过呼吸道传播引起全球流行病。当务之急是制定适当的干预措施，保护个人免受未来大流行病的影响。这项研究提出了一种鼻喷雾剂，通过层层自组装（LBL）方法在细胞表面形成多糖“盔甲”，以最大限度地降低病毒感染的风险。鼻喷雾剂有两个独立的成分：壳聚糖和藻酸盐。利用静电相互作用，交替吸入两种多糖能够组装屏障，减少病毒进入细胞的摄取。结果表明，该方法对细胞无明显损伤，并赋予细胞抵抗腺病毒和SARS-CoV-2假病毒感染的能力。这种方法可能是一种潜在的预防策略，用于保护呼吸道免受多种病毒的侵害，特别是即将到来的SARS-CoV-2变体。
Airborne pathogens, such as the world-spreading severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), cause global epidemics via transmission through the respiratory pathway. It is of great urgency to develop adequate interventions that can protect individuals against future pandemics. This study presents a nasal spray that forms a polysaccharide “armor” on the cell surface through the layer-by-layer self-assembly (LBL) method to minimize the risk of virus infection. The nasal spray has two separate components: chitosan and alginate. Harnessing the electrostatic interaction, inhaling the two polysaccharides alternatively enables the assembly of a barrier that reduces virus uptake into the cells. The results showed that this approach has no obvious cellular injury and endows cells with the ability to resist the infection of adenovirus and SARS-CoV-2 pseudovirus. Such a method can be a potential preventive strategy for protecting the respiratory tract against multiple viruses, especially the upcoming SARS-CoV-2 variants.