Initiation of SV40 DNA replication in vitro: analysis of the role played by sequences flanking the core origin on initial synthesis events.
Initiation of SV40 DNA replication in vitro: analysis of the role played by sequences flanking the core origin on initial synthesis events.
复制标题
SV40 DNA 体外复制的启动：分析核心起点侧翼序列对初始合成事件所起的作用。
DOI：
10.1006/viro.1996.8347
复制
发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Mello,C
中科院分区：
文献类型：
--
作者：
Bullock,PA;Joo,WS;Sreekumar,KR;Mello,C
Replication initiation events are suppressed over the SV40 core originin vitro;they are also greatly reduced over sequences flanking the origin which contain binding sites for several transcription factors. To address the biochemical basis for the gap in initiation events over the flanking sequences, initial synthesis events have been characterized on templates lacking these sequences. Herein, it is demonstrated that previously functional initiation sites are nearly inactive when moved to positions that are proximal to the core origin. Thus, the gap in initiation events depends, in part, on the proximity of the initiation sites to the SV40 core origin. Additional experiments demonstrate that removal of the flanking sequences had little or no effect on DNA unwinding or on the efficiency of initiation of DNA synthesisin vitro.These results indicate that, under ourin vitroconditions, initiation of SV40 DNA synthesis is not enhanced by binding of transcription factors to the flanking sequences.
