Biological performances of collagen-based scaffolds tor vascular tissue engineering
Biological performances of collagen-based scaffolds tor vascular tissue engineering
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DOI：
10.1016/j.biomaterials.2005.05.052
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发表时间：
2005-12-01
期刊：
影响因子：
14
通讯作者：
Mantovani, D
中科院分区：
文献类型：
--
作者：
Boccafoschi, F;Habermehl, J;Mantovani, D
胶原蛋白在生物医学上有着广泛的应用，是血管组织工程中一种有效的替代支架材料。在这项工作中，从中和的酸溶性溶液中制备重组胶原膜，用于随后的血液相容性和细胞活力性能测定。首先，进行无血红蛋白、血栓造影和血小板粘附试验，以研究血液接触性能。其次，将标本分别植入内皮细胞和平滑肌细胞，通过MTT和SEM检测细胞活力。结果表明，中和后的酸溶性I型胶原膜不增强血液凝固，不改变血液的正常粘弹性，轻微激活血小板粘附和聚集。细胞培养表明，样品是足够的基质，以支持内皮细胞和平滑肌细胞的粘附和增殖。(c) 2005 Elsevier Ltd版权所有。
Collagen is widely used for biomedical applications and it could represent a valid alternative scaffold material for vascular tissue engineering. In this work, reconstituted collagen films were prepared from neutralized acid-soluble solutions for subsequent haemocompatibility and cell viability performance assays. First, haemoglobin-free, thrombelastography and platelet adhesion tests were performed in order to investigate the blood contact performance. Secondly, specimens were seeded with endothelial cells and smooth muscle cells, and cell viability tests were carried out by MTT and SEM. Results show that neutralized acid-soluble type I collagen films do not enhance blood coagulation, do not alter normal viscoelastic properties of blood and slightly activate platelet adhesion and aggregation. Cell culture shows that the samples are adequate substrates to support the adhesion and proliferation of endothelial and smooth muscle cells. (c) 2005 Elsevier Ltd. All rights reserved.