Detection of Anaplasma platys in dogs and Rhipicephalus sanguineus group ticks by a quantitative real-time PCR
Detection of Anaplasma platys in dogs and Rhipicephalus sanguineus group ticks by a quantitative real-time PCR
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DOI：
10.1016/j.vetpar.2014.06.023
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发表时间：
2014-09-15
影响因子：
2.6
通讯作者：
Otranto, Domenico
中科院分区：
文献类型：
--
作者：
Ramos, Rafael Antonio Nascimento;Latrofa, Maria Stefania;Otranto, Domenico
鸭无原体是一种专性细胞内，蜱传播的病原体的狗，它导致犬传染性循环血小板减少症（CICT）。只提出了血根头蜱群蜱的媒介作用，但缺乏明确的证据。本研究旨在通过实时荧光定量PCR法检测和定量感染犬及其蜱的platys DNA。从2009年3月至5月，收集了居住在cit流行地区的狗的血液和蜱虫样本并进行了分子分析。蜱螨若虫(3.5 × 10（-2) +/- 2.5 × 10(-2)）与成虫(雌性：1.2 × 10（-1) +/- 1.1 × 10(-1)）的细菌载量差异有统计学意义（p < 0.05）；雄性：9.3 × 10(-2) +/- 1.2 × 10(-2))，介于未充血蜱(1.1 × 10（-1) +/- 2.8 × 10(-2)）和部分或完全充血蜱(1.9 × 10（-1) +/- 2.7 × 10(-2)）之间。阳性犬(1.1 × 10（-1) +/- 0.6 × 10(-1)）和阴性犬(1.2 × 10（-1) +/- 0.3 × 10(-1)）蜱虫数量差异无统计学意义（p < 0.05）。阳性犬的平均细菌载量低于各自蜱（p < 0.05）。本研究为推测I型鼻头虫是该病原体的传播媒介提供了间接证据。(C) 2014 Elsevier B.V.版权所有
Anaplasma platys is an obligate intracellular, tick-borne pathogen of dogs, which causes canine infectious cyclic thrombocytopenia (CICT). The vector role of Rhipicephalus sanguineus group ticks has been only suggested, but definitive evidence is lacking. This study aimed to detect and quantify A. platys DNA in infected dogs and in their respective ticks through a quantitative real-time PCR assay. From March to May 2009, blood and tick samples from dogs residing in a CICT-endemic area were collected and molecularly analysed. Differences (p < 0.05) were detected in the bacterial load between tick nymphs (3.5 x 10(-2) +/- 2.5 x 10(-2)) and adults (female: 1.2 x 10(-1) +/- 1.1 x 10(-1); male: 9.3 x 10(-2) +/- 1.2 x 10(-2)) and between unengorged (1.1 x 10(-1) +/- 2.8 x 10(-2)) and partially or fully engorged ticks (1.9 x 10(-1) +/- 2.7 x 10(-2)). No difference was found between ticks collected from positive (1.1 x 10(-1) +/- 0.6 x 10(-1)) and negative (1.2 x 10(-1) +/- 0.3 x 10(-1)) dogs (p > 0.05). The mean bacterial load detected in positive dogs was lower than that in their respective ticks (p > 0.05). This study provides circumstantial evidence of the putative role of Rhipicephalus sp. I as a vector of this pathogen. (C) 2014 Elsevier B.V. All rights reserved.