Cell viability of aortic allografts after long-term cryopreservation and clinical application to aortic root replacement in a patient with aortitis.
Cell viability of aortic allografts after long-term cryopreservation and clinical application to aortic root replacement in a patient with aortitis.
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DOI：
10.1016/0967-2109(95)90902-h
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发表时间：
1995-04-01
期刊：
影响因子：
--
通讯作者：
Oka, T
中科院分区：
文献类型：
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作者：
Motomura, N;Eishi, K;Oka, T
建立组织冷冻保存系统的关键点是在较长时间内保持细胞活力。本文报道了组织细胞在作者研制的冻存系统中的存活情况，并首次应用于临床。组织培养方法证明，95%的大鼠心瓣膜（21/22个标本）和88%的人心脏瓣膜（7/8个）分别在冷冻保存6.4个月和5.8个月后存活。第一个临床病例，一位54岁的男性，主动脉瓣置换术和并发症的肠炎，进行了成功的主动脉根部置换术。尽管同种异体移植物在动脉炎病例中的使用存在争议，但这种方法被认为是合理的，因为自体主动脉瓣环和插入的瓣膜之间的机械应力可以通过同种异体移植物的主动脉根部置换来降低。
A key point in the establishment of a tissue cryopreservation system is to maintain cell viability for an extended period. The viability of the tissue cells in the authors' cryopreservation system was examined, and the first clinical application reported. The tissue culture method demonstrated 95% of rat aortas (21 of 22 specimens) and 88% of human cardiac valves (seven of eight) to be viable after 6.4 months and 5.8 months of cryopreservation, respectively. The first clinical case, a 54-year-old man with aortic valve replacement and complications of aortitis, underwent successful aortic root replacement. Whereas the use of allografts in aortitis cases is controversial, such an approach was assumed reasonable because the mechanical stress between the native aortic annulus and the inserted valve could be reduced by aortic root replacement with the allograft.