Transport of peptide-MHC class II complexes in developing dendritic cells
Transport of peptide-MHC class II complexes in developing dendritic cells
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DOI：
10.1126/science.288.5465.522
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发表时间：
2000-04-21
期刊：
影响因子：
56.9
通讯作者：
Mellman, I
中科院分区：
文献类型：
--
作者：
Turley, SJ;Inaba, K;Mellman, I
主要组织相容性复合体II类(MHC II)分子捕获内吞途径中的多肽，以产生T细胞受体(TCR)配体。未成熟树突状细胞(DC)在溶酶体中隔离完整的抗原，在诱导DC成熟时将抗原加工并转化为多肽-MHC II复合体。然后，这些复合体聚集在独特的、非溶酶体的MHC II+小泡中，这些小泡似乎迁移到细胞表面。尽管囊泡不包括可溶性溶酶体内容物和抗原处理机制，但许多囊泡包含MHC I和B7共刺激分子。到达细胞表面后，MHC和共刺激分子仍然聚集在一起。因此，DC对多肽-MHC II复合体的转运不仅完成了从晚期内吞细胞室到质膜的转移，而且以选择性地浓缩TCR配体和共刺激分子用于T细胞接触的方式完成了这一过程。
Major histocompatibility complex class II (MHC II) molecules capture peptides within the endocytic pathway to generate T cell receptor (TCR) ligands. Immature dendritic cells (DCs) sequester intact antigens in Lysosomes, processing and converting antigens into peptide-MHC II complexes upon induction of DC maturation. The complexes then accumulate in distinctive, nonlysosomal MHC II+ vesicles that appear to migrate to the cell surface. Although the vesicles exclude soluble lysosomal contents and antigen-processing machinery, many contain MHC I and B7 costimulatory molecules. After arrival at the cell surface, the MHC and costimulatory molecules remain clustered. Thus, transport of peptide-MHC II complexes by DCs not only accomplishes transfer from late endocytic compartments to the plasma membrane, but does so in a manner that selectively concentrates TCR ligands and costimulatory molecules for T cell contact.