Methanococcoides vulcani sp nov., a marine methylotrophic methanogen that uses betaine, choline and N,N-dimethylethanolamine for methanogenesis, isolated from a mud volcano, and emended description of the genus Methanococcoides
Methanococcoides vulcani sp nov., a marine methylotrophic methanogen that uses betaine, choline and N,N-dimethylethanolamine for methanogenesis, isolated from a mud volcano, and emended description of the genus Methanococcoides
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DOI：
10.1099/ijs.0.058289-0
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发表时间：
2014-06-01
影响因子：
2.8
通讯作者：
Toffin, Laurent
中科院分区：
文献类型：
--
作者：
L'Haridon, Stephane;Chalopin, Morgane;Toffin, Laurent
从那不勒斯泥火山收集的覆盖着橙色微生物垫的深层沉积物样品中分离出一种新型、严格厌氧、甲基营养型海洋产甲烷菌菌株 SLH33(T)。 SLH33(T)菌株的细胞是革兰氏染色阴性、活动的、不规则的单个球菌。细胞利用三甲胺、二甲胺、一甲胺、甲醇、甜菜碱、N,N-二甲基乙醇胺和胆碱（N,N,N-三甲基乙醇胺）作为生长和产甲烷的底物。最适生长温度为30℃；在 pH 7.0、0.5 M Na+ 存在下获得最大生长速率。菌株SLH33(T)的DNA G+C含量为43.4mol％。基于 16S rRNA 基因序列的系统发育分析将菌株 SLH33(T) 归入甲烷球菌属。该新分离株与甲基球菌TMA-10(T)关系最密切（16S rRNA基因序列相似性为98.8%），但与伯托甲烷球菌DSM 6242(T) (97.6%)和阿拉斯加甲烷球菌AK-5(T) (97.6%)关系较远。 DNA DNA 杂交研究表明，菌株 SLH33(T) 代表了一个新物种，因为它与甲基甲烷球菌 TMA-10(T) 的 DNA DNA 相关性不到 16%。名称：Methanococcoides vulcani sp。十一月提议针对该新物种，以菌株 SLH33(T) (=DSM 26966(T)=JCM 19278(T)) 作为模式菌株。还提出了对甲烷球菌属的修订描述。
A novel, strictly anaerobic, methylotrophic marine methanogen, strain SLH33(T), was isolated from deep sediment samples covered by an orange microbial mat collected from the Napoli Mud Volcano. Cells of strain SLH33(T) were Gram-stain-negative, motile, irregular cocci that occurred singly. Cells utilized trimethylamine, dimethylamine, monomethylamine, methanol, betaine, N,N-dimethylethanolamine and choline (N,N,N-trimethylethanolamine) as substrates for growth and methanogenesis. The optimal growth temperature was 30 degrees C; maximum growth rate was obtained at pH 7.0 in the presence of 0.5 M Na+. The DNA G+C content of strain SLH33(T) was 43.4 mol%. Phylogenetic analyses based on 16S rRNA gene sequences placed strain SLH33(T) within the genus Methanococcoides. The novel isolate was related most closely to Methanococcoides methylutens TMA-10(T) (98.8% 16S rRNA gene sequence similarity) but distantly related to Methanococcoides burtonii DSM 6242(T) (97.6%) and Methanococcoides alaskense AK-5(T) (97.6%). DNA DNA hybridization studies indicated that strain SLH33(T) represents a novel species, given that it shared less than 16% DNA DNA relatedness with Methanococcoides methylutens TMA-10(T). The name Methanococcoides vulcani sp. nov. is proposed for this novel species, with strain SLH33(T) (=DSM 26966(T)=JCM 19278(T)) as the type strain. An emended description of the genus Methanococcoides is also proposed.