Estrogen reduces endoplasmic reticulum stress to protect against glucotoxicity induced-pancreatic β-cell death
Estrogen reduces endoplasmic reticulum stress to protect against glucotoxicity induced-pancreatic β-cell death
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DOI：
10.1016/j.jsbmb.2013.09.018
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发表时间：
2014-01-01
影响因子：
4.1
通讯作者：
Yenchitsomanus, Pa-thai
中科院分区：
文献类型：
--
作者：
Kooptiwut, Suwattanee;Mahawong, Pitchnischa;Yenchitsomanus, Pa-thai
Estrogen can improve glucose homeostasis not only in diabetic rodents but also in humans. However, the molecular mechanism by which estrogen prevents pancreatic beta-cell death remains unclear. To investigate this issue, INS-1 cells, a rat insulinoma cell line, were cultured in medium with either 11.1 mM or 40 mM glucose in the presence or the absence of estrogen. Estrogen significantly reduced apoptotic beta-cell death by decreasing nitrogen-induced oxidative stress and the expression of the ER stress markers GRP 78, ATF6, P-PERK, PERK, uXBP1, sXBP1, and CHOP in INS-1 cells after prolonged culture in medium with 40 mM glucose. In contrast, estrogen increased the expression of survival proteins, including sarco/endoplasmic reticulum Ca2+ ATPase (SERCA-2), Bcl-2, and P-p38, in INS-1 cells after prolonged culture in medium with 40 mM glucose. The cytoprotective effect of estrogen was attenuated by addition of the estrogen receptor (ER alpha and ER beta) antagonist ICI 182,780 and the estrogen membrane receptor inhibitor G15. We showed that estrogen decreases not only oxidative stress but also ER stress to protect against 40 mM glucose-induced pancreatic beta-cell death. (C) 2013 Elsevier Ltd. All rights reserved.
