Microbial community analysis of thermophilic contact oxidation process by using ribosomal RNA approaches and the quinone profile method.
Microbial community analysis of thermophilic contact oxidation process by using ribosomal RNA approaches and the quinone profile method.
复制标题
使用核糖体 RNA 方法和醌谱方法对嗜热接触氧化过程进行微生物群落分析。
DOI：
10.1016/s0043-1354(01)00225-1
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发表时间：
2002
期刊：
影响因子：
12.8
通讯作者：
T. Matsuo
中科院分区：
文献类型：
--
作者：
F. Kurisu;H. Satoh;T. Mino;T. Matsuo
采用多种非培养方法对实验室规模的高温好氧污水处理反应器的微生物群落结构进行了分析。醌谱法提供了呼吸醌分子种类的化学分析，其对应于细菌群。变性梯度凝胶电泳（DGGE）的PCR扩增的16 S rDNA部分序列（PCR-DGGE）阐明了物种水平上的群落变化，因为DGGE可以分离不同序列的DNA片段。细菌细胞的某些系统发育组可以通过荧光原位杂交（FISH）标记。醌谱显示主要存在MK-7。PCR-DGGE分析表明，在稳定期，群落的组成成分没有发生变化。FISH结果显示迟缓芽孢杆菌和B的亲缘关系存在。在相当大的比例中的热封。该群落以芽孢杆菌科为主，在35°C ~ 60°C的温度波动范围内均出现专性嗜热芽孢杆菌和中温芽孢杆菌。这些培养独立的方法相结合，揭示了社区精确到足以评估反应器的性能。
Microbial community structure of a lab scale thermophilic aerobic wastewater treatment reactor was analyzed by a combination of culture-independent methods. Quinone profile method provides for chemical analysis of respiratory quinone molecular species, which corresponds to bacterial groups. Denaturing gradient gel electrophoresis (DGGE) of PCR-amplified 16S rDNA partial sequences (PCR-DGGE) clarifies community changes at species level, as DGGE can separate DNA fragments of different sequences. Certain phylogenetic groups of bacterial cells can be labeled by fluorescence in situ hybridization (FISH). Quinone profile showed a predominant presence of MK-7. PCR-DGGE revealed that constituents of the community were unchanged during the stable phase. FISH demonstrated the existence of the relatives of Bacillus lentus and B. thermocloacae in considerable proportions. The community was mainly composed of Bacillaceae, and obligate thermophilic and mesophilic Bacillus appeared in spite of the temperature fluctuation from 35°C to 60°C. The combination of these culture-independent methods revealed the community precisely enough to evaluate the reactor performance.