Genome-wide identification and expression profiling of ankyrin-repeat gene family in maize
Genome-wide identification and expression profiling of ankyrin-repeat gene family in maize
复制标题
玉米锚蛋白重复基因家族的全基因组鉴定和表达谱
DOI：
10.1007/s00427-013-0447-7
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发表时间：
2013-09-01
影响因子：
2.4
通讯作者：
Zhu, Suwen
中科院分区：
文献类型：
--
作者：
Jiang, Haiyang;Wu, Qingqing;Zhu, Suwen
锚蛋白重复序列（ankyrin repeats， ANK）基因家族成员编码的ANK结构域在多种生物中普遍存在，在细胞生长发育过程中发挥重要作用，如细胞-细胞信号转导和细胞周期调控。近年来，人们对拟南芥和水稻的ANK基因家族进行了全基因组鉴定和进化分析。然而，对整个玉米基因组中的ANK基因知之甚少。在本研究中，我们描述了玉米（ZmANK）中71个ANK基因的鉴定和结构特征。然后，对zmankgenes家族进行了全面的生物信息学分析，包括系统发育、结构域和基序分析、染色体定位、内含子/外显子结构模式、基因复制和表达谱。结构域组成分析表明，zmankgenes形成了10个亚家族。在zmankgenes中鉴定出5个串联重复和14个片段重复。此外，我们对拟南芥、水稻和玉米的ANK基因家族进行了比较分析，ZmANKs与OsANKs的配对关系比与AtANKs的配对关系更密切。最后进行表达谱分析。zmankgenes的41个成员拥有EST序列记录。对这41个zmank基因的半定量表达和微阵列数据分析表明，zmank基因表现出多种表达模式，表明zmank基因家族的功能多样化。研究结果将为探索ANK基因在玉米中的表达和功能提供重要的见解。
Members of the ankyrin repeats (ANK) gene family encode ANK domain that are common in diverse organisms and play important roles in cell growth and development, such as cell-cell signal transduction and cell cycle regulation. Recently, genome-wide identification and evolutionary analyses of the ANK gene family have been carried out inArabidopsisand rice. However, little is known regarding the ANK genes in the entire maize genome. In this study, we described the identification and structural characterization of 71 ANK genes in maize (ZmANK). Then, comprehensive bioinformatics analyses ofZmANKgenes family were performed including phylogenetic, domain and motif analysis, chromosomal localization, intron/exon structural patterns, gene duplications and expression profiling. Domain composition analyses showed thatZmANKgenes formed ten subfamilies. Five tandem duplications and 14 segmental duplications were identified inZmANKgenes. Furthermore, we took comparative analysis of the total ANK gene family inArabidopsis, rice and maize, ZmANKs were more closely paired with OsANKs than with AtANKs. At last, expression profile analyses were performed. Forty-one members ofZmANKgenes held EST sequences records. Semi-quantitative expression and microarray data analysis of these 41ZmANKgenes demonstrated thatZmANKgenes exhibit a various expression pattern, suggesting that functional diversification ofZmANKgenes family. The results will present significant insights to explore ANK genes expression and function in future studies in maize.