Systemic estradiol administration to ovariectomized rats facilitates thermoregulatory behavior in a cold environment
Systemic estradiol administration to ovariectomized rats facilitates thermoregulatory behavior in a cold environment
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卵巢切除大鼠全身雌二醇促进寒冷环境中的体温调节行为
DOI：
10.1016/j.brainres.2017.06.009
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发表时间：
2017
期刊：
影响因子：
2.9
通讯作者：
Kazunari Yuri
中科院分区：
文献类型：
--
作者：
Yuki Uchida;Kei Nagashima;Kazunari Yuri
在寒冷的环境中，大鼠将尾巴放在身体下面（藏尾行为），这是体温调节的行为指标。本研究的目的是阐明雌二醇（E2）的影响，对尾部隐藏的行为和神经活动的免疫组织化学评估。将去卵巢大鼠背部皮下植入含或不含E2的硅橡胶管（E2（-）和E2（+）组），并暴露于27 °C、16 °C和10 °C下2 h，连续测量体温（Tb）、尾部皮肤温度（Ttail）和行为。计数海马、次级躯体感觉皮质、内侧视前核、视旁核、杏仁核和臂旁外侧核的cFos免疫反应阳性细胞。E2（-）和E2（+）组之间T和Ttail无差异。在16 °C时，E2（+）组的藏尾行为持续时间和开始时间均大于E2（-）组。在16 °C条件下，E2（-）组大鼠海马CA 1区cFos-IR细胞数量多于E2（+）组。E2可能调节雌性大鼠在16 °C下的藏尾行为，并且脑岛可能参与了该反应。
Rats place their tails underneath their bodies in the cold (tail-hiding behavior), which is a behavioral indicator of thermoregulation. The aim of the present study was to elucidate the effect of estradiol (E2) on tail-hiding behavior and neural activity assessed by immunohistochemistry. Ovariectomized rats were implanted with a silastic tube with or without E2underneath the dorsal skin (E2(−) and E2(+) groups), and exposed to 27 °C, 16 °C, and 10 °C for 2 h with continuous body temperature (Tb), tail skin temperature (Ttail), and behavioral measurements. cFos immunoreactive (cFos-IR) cells in the insula, secondary somatosensory cortex, medial preoptic nucleus, parastrial nucleus, amygdala, and lateral parabrachial nucleus were counted. Tband Ttailwere not different between the E2(−) and E2(+) groups. At 16 °C, the duration and the onset of tail-hiding behavior in the E2(+) group were greater than that in the E2(−) group. The number of cFos-IR cells in the insula of the E2(−) group was greater than that of the E2(+) group in rats kept at 16 °C. E2might modulate tail-hiding behavior of female rats at 16 °C, and the insula may be involved in the response.