The past, present and future of enzyme measurements using surface enhanced Raman spectroscopy
The past, present and future of enzyme measurements using surface enhanced Raman spectroscopy
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DOI：
10.1039/c0sc00226g
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发表时间：
2010-01-01
期刊：
影响因子：
8.4
通讯作者：
Graham, Duncan
中科院分区：
文献类型：
--
作者：
Larmour, Iain A.;Faulds, Karen;Graham, Duncan
准确和灵敏地测量特定酶活性的能力是现代化学许多方面的核心，当与基于纳米科学的新方法相结合时，为推进其他科学学科提供了重要的机会。我们回顾了表面增强共振拉曼散射（SERRS）检测酶的发展，从最初的直接光谱的酶，底物/产品和抑制剂吸附到金属胶体，到目前的方法测量酶的活性，通过记录的SERRS光谱的产品，这是唯一的“打开”酶活性后。发展集中在改进模块化掩蔽SERRS基板，这是由特定的酶揭开，也进行了审查。最后，我们列出了SERRS酶分析领域内剩余的重大挑战，包括单分子检测，体内研究和增加多重筛选进化的酶库。
The ability to accurately and sensitively measure the activity of specific enzymes is central to many aspects of modern chemistry and when combined with new nanoscience based approaches, offers significant opportunities for advancing other scientific disciplines. We review the development of surface enhanced resonance Raman scattering (SERRS) for the detection of enzymes, from the initial direct spectroscopy of enzymes, substrate/product and inhibitors adsorbed onto metallic colloids, to the current approach of measuring enzymatic activity by recording the SERRS spectra of a product which is only 'switched on' after enzyme activity. Developments focused on improvements to modular masked SERRS substrates, which are unmasked by specific enzymes, are also reviewed. Finally, we set out the remaining grand challenges within the area of enzymatic analysis by SERRS which include single molecule detection, in vivo studies and increased multiplexing for screening of evolved enzyme libraries.