Multilocus genotyping of Giardia duodenalis and Enterocytozoon bieneusi in dairy and native beef (Qinchuan) calves in Shaanxi province, northwestern China
Multilocus genotyping of Giardia duodenalis and Enterocytozoon bieneusi in dairy and native beef (Qinchuan) calves in Shaanxi province, northwestern China
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DOI：
10.1007/s00436-016-4908-6
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发表时间：
2016-03-01
影响因子：
2
通讯作者：
Zhao, G. H.
中科院分区：
文献类型：
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作者：
Wang, X. T.;Wang, R. J.;Zhao, G. H.
贾第鞭毛虫和比氏肠细胞虫是两种常见的寄生于动物和人类肠道上皮的原虫。犊牛已被确定为这两种病原体的重要宿主，但中国犊牛中这两种病原体的数据有限。本研究采用多位点基因分型（MLST）技术，对陕西省奶牛和本地肉牛（秦川牛）犊牛的寄生虫感染率和聚集/基因型进行了分析。在371份犊牛粪样中（其中乳犊牛198头，秦川犊牛173头），G. sperialis和E.比纽氏综合征为18.87（70/371）和19.68%（73/371）。人畜共患G.在奶犊牛和秦川犊牛中发现了动物适应性组合E和动物适应性组合A。十七，八，五，和两个G。在磷酸丙糖异构酶（tpi）、β-贾第丁（bg）、谷氨酸脱氢酶（gdh）和小亚基核糖体RNA（SSU-rRNA）基因座上检测到牛链球菌亚型，其中在tpi和bg基因座上检测到5种和2种新亚型，形成25种多重基因型（MLG）（在奶牛和秦川犊牛中分别为15种和11种）。73份E.结果表明，在核糖体RNA内转录间隔区（ITS）基因座上发现了5种ITS基因型，包括3种已知的人畜共患病基因型（I、J、CHN 1）和2种新的基因型（CSX 1和CSX 2）。对3个微卫星位点（MS 1、MS 3、MS 7）和1个小卫星位点（MS 4）进行MLST分析，在MS 1、MS 3、MS 4和MS 7位点分别检测到6、2、2和2种基因型，形成10个MLG（奶牛和秦川犊牛分别为7和4个）。这些结果揭示了G. sperialis和E. bieneusi在陕西省犊牛中的流行情况以及这两种病原体在陕西省犊牛中的人畜共患病潜力。
Giardia duodenalis and Enterocytozoon bieneusi are two common protozoa that parasitize the intestinal epithelium of animals and humans. Calves have been identified as important reservoirs of these two pathogens, but limited data is available for these two pathogens in calves in China. In the present study, the prevalence and assemblages/genotypes of both parasites in calves of dairy and native beef (Qinchuan) cattle in Shaanxi province, northwestern China, were analyzed using multilocus genotyping (MLST). Of 371 fecal samples collected from calves (including 198 dairy calves and 173 Qinchuan calves), the respective overall prevalence of G. duodenalis and E. bieneusi was 18.87 (70 of 371) and 19.68 % (73 of 371). Both the zoonotic G. duodenalis assemblage A and animal adapted assemblage E were found in dairy and Qinchuan calves. Seventeen, eight, five, and two G. duodenalis subtypes were detected at the triose phosphate isomerase (tpi), beta-giardin (bg), glutamate dehydrogenase (gdh), and small subunit ribosomal RNA (SSU-rRNA) loci, with five and two novel subtypes detected at the tpi and bg loci, forming 25 multiple genotypes (MLGs) (15 and 11 in dairy and Qinchuan calves, respectively). Of 73 samples that were positive for E. bieneusi at the ribosomal RNA internal transcribed spacer (ITS) locus, five ITS genotypes were found, including three known zoonotic genotypes (I, J, CHN1) and two novel genotypes (CSX1 and CSX2). MLST analysis of three microsatellite loci (MS1, MS3, MS7) and one minisatellite locus (MS4) detected six, two, two, and two genotypes at the MS1, MS3, MS4, and MS7 loci, respectively, forming ten MLGs (seven and four in dairy and Qinchuan calves, respectively). These results indicate complex population structures of G. duodenalis and E. bieneusi in calves in Shaanxi province and the zoonotic potential of these two pathogens in calves in this province.