Neural cell fate analysis in zebrafish using olig2 BAC transgenics.
Neural cell fate analysis in zebrafish using olig2 BAC transgenics.
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DOI：
10.1023/b:mics.0000006847.09037.3a
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发表时间：
2003-01-01
期刊：
影响因子：
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通讯作者：
Appel, Bruce
中科院分区：
文献类型：
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作者：
Shin, Jimann;Park, Hae-Chul;Appel, Bruce
我们在这里描述了使用BAC克隆生产转基因斑马鱼报告细胞系的简单方法。BAC克隆的使用有助于产生有用的转基因，因为它们含有的大量基因组DNA增加了报告基因表达将被适当调控的可能性。结合活胚胎图像分析的最新进展，这种策略有可能大大推进发育过程中神经细胞行为的研究。
We describe here simple methods for producing transgenic zebrafish reporter lines using BAC clones. The use of BAC clones facilitates creation of useful transgenics as the large amounts of genomic DNA they contain increase the likelihood that reporter gene expression will be properly regulated. Combined with recent advances in live embryo image analysis, this strategy has the potential to greatly advance the investigation of neural cell behavior during development.