Endothelin-1, α-Klotho, 25(OH) Vit D levels and severity of disease in scleroderma patients
Endothelin-1, α-Klotho, 25(OH) Vit D levels and severity of disease in scleroderma patients
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DOI：
10.1007/s00296-017-3797-z
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发表时间：
2017-10-01
影响因子：
4
通讯作者：
Mahdavi, Aida Malek
中科院分区：
文献类型：
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作者：
Hajialilo, Mehrzad;Noorabadi, Parisa;Mahdavi, Aida Malek
考虑到内皮素-1 (ET-1)在硬皮病发展过程中组织重塑和纤维化中的作用，以及α-Klotho在钙质沉着和/或内皮细胞损伤发病机制中的作用及其与疾病严重程度的相关性，本研究旨在评估局限性和弥漫性硬皮病患者与健康受试者相比的血清ET-1、α-Klotho和25(OH)维生素D水平。在这项横断面研究中，纳入了 60 名符合 ACR/EULAR 2013 标准的硬皮病患者和 60 名年龄和性别匹配的健康对照。对患者进行临床检查并评估 Medsger 严重程度量表。使用 ELISA 试剂盒测量血清 ET-1、可溶性 α-Klotho 和 25(OH)D-3 水平。患者和对照的平均+/- SD年龄分别为46.2 +/- 9.6和47.2 +/- 7.0岁。与健康对照相比，SSc 患者的血清 ET-1 显着升高 (p = 0.001)；而患者血清 α-Klotho 和 25(OH)D-3 显着降低 (p = 0.001)。患者最常见的受累器官是皮肤、肺、周围血管和胃肠系统，受累严重程度以轻度和/或中度为主。根据不同器官受累的严重程度，血清 ET-1 和 α-Klotho 水平没有显着差异（p > 0.05）。是否存在钙质沉着与 ET-1、α-Klotho 和 25(OH)D-3 水平自身抗体的阴性或阳性之间没有显着相关性。尽管我们的研究显示，与健康对照相比，SSc 患者的血清 ET-1 水平较高，血清 α-Klotho 水平较低，但他们的血清水平与器官受累的严重程度之间没有任何显着相关性。
Considering the role of endothelin-1 (ET-1) in tissue remodeling and fibrosis during the development of scleroderma as well as the effect of alpha-Klotho in pathogenesis of calcinosis and/or endothelial cell injury and its correlation with severity of disease, this study aimed to evaluate serum ET-1, alpha-Klotho and 25(OH) vitamin D levels in patients with limited and diffuse scleroderma compared to healthy subjects. In this cross-sectional study, 60 scleroderma patients according to the ACR/EULAR 2013 criteria and 60 age- and sex-matched healthy controls were included. In patients, clinical examination was performed and Medsger severity scale was assessed. Serum ET-1, soluble alpha-Klotho and 25(OH)D-3 levels were measured using ELISA kits. The mean +/- SD age of patients and controls was 46.2 +/- 9.6 and 47.2 +/- 7.0 years, respectively. Compared to healthy controls, serum ET-1 was significantly higher in SSc patients (p = 0.001); whilst serum alpha-Klotho and 25(OH)D-3 were significantly lower in patients (p = 0.001). The most common organs involved in patients were skin, lung, peripheral vascular and gastrointestinal system and the severity of involvement was mainly mild and/or moderate. There were no significant differences in serum ET-1 and alpha-Klotho levels according to the severity of different organ involvement (p > 0.05). There was no significant correlation between presence or absence of calcinosis and negative or positivity of auto-antibodies with ET-1, alpha-Klotho and 25(OH)D-3 levels. Although our study revealed higher serum ET-1 and lower serum alpha-Klotho levels in SSc patients compared to healthy controls, there were not any significant correlations between their serum levels with severity of organ involvement.