Serotonin involvement in the electroacupuncture- and moxibustion-induced gastric emptying in rats
Serotonin involvement in the electroacupuncture- and moxibustion-induced gastric emptying in rats
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DOI：
10.1016/j.physbeh.2004.07.003
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发表时间：
2004-10-15
影响因子：
2.9
通讯作者：
Mello, LEAM
中科院分区：
文献类型：
--
作者：
Sugai, GCM;Freire, AD;Mello, LEAM
目的：电针和艾灸刺激对大鼠胃肠运动均有兴奋作用。肠间神经丛和粘膜下神经丛的5-羟色胺能神经元是胃肠生理的主要参与者。在此，我们比较了针灸和艾灸刺激特定穴位对胃肠运动的影响以及5-羟色胺(5-HT)在其中的作用。方法：研究了25个玻璃小球在5-羟色胺抑制剂(对氯苯丙氨酸[PCPA])处理后对胃肠运动的影响，以分析5-HT对大鼠胃肠运动的影响。针刺刺激ST-36、Sp-6(足三里)、Sp-6(三阴交)，或人-10(下湾)、人-12(中环)、ST-25(天俞)，艾灸。结果：与假刺激动物相比，刺激后肢(ST-36、Sp-6)和腹部(人-10、人-12、ST-25)穴位可有效地促进胃排空。PCPA对动物后肢穴位的针刺刺激反应和腹穴艾灸刺激反应均无明显影响。结论：艾灸腹穴和电针后肢需要完整的5-羟色胺能通路。此外，我们认为5-羟色胺的这种参与是针刺对大鼠胃排空的中介效应的一个普遍特征。(C)2004 Elsevier Inc.保留所有权利。
Objective: Electroacupuncture (EA) as well as moxibustion stimulation has been reported to produce an excitatory effect on the gastrointestinal motility of the rat. Serotonergic neurons of the mioenteric and submucous plexus are major participants in the gastrointestinal physiology. Here, we compared the outcomes of the stimulation of a specific set of acupoints with either acupuncture or moxibustion on the gastrointestinal motility and the role of serotonin (5-HT) in this effect.Methods: To analyze the role of 5-HT on the gastrointestinal motility of the rat, we studied the flow of 25 glass beads administered to the stomach, after treatment of the animals with a serotonin inhibitor (para-chlorophenylalanine [pCPA]). Acupuncture stimulation was performed on acupoints St-36 (Zusanli) and Sp-6 (Sanyinjiao), with electrical stimulation, or on acupoints Ren-10 (Xiawan), Ren-12 (Zhongwan) and St-25 (Tianshu), with moxibustion. Animals subjected to sham stimulation were used as controls in addition to naive, unstimulated animals.Results: Stimulation of the hind limb (St-36 and Sp-6) and abdominal (Ren-10, Ren-12, St-25) acupoints resulted in effective gastric emptying, as compared with sham-stimulated animals. Pretreatment of animals with pCPA abolished either the response provided by acupuncture stimulation in animal groups subjected to hind limb acupoints or the response provided by moxibustion stimulation in abdominal acupoints.Conclusion: Our data suggest that moxibustion in the abdominal points and EA in the hind limb require an intact serotonergic pathway. In addition, we suggest that this involvement of serotonin is a general feature of the mediated effects of acupuncture on gastric emptying of the rat. (C) 2004 Elsevier Inc. All rights reserved.