Gata3 is required for early morphogenesis and Fgf10 expression during otic development
Gata3 is required for early morphogenesis and Fgf10 expression during otic development
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DOI：
10.1016/j.mod.2006.04.007
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发表时间：
2006-06-01
影响因子：
2.6
通讯作者：
Salminen, Marjo
中科院分区：
文献类型：
--
作者：
Lillevali, Kersti;Haugas, Maarja;Salminen, Marjo
内耳由一个诱导的表面外胚层基板发育而成，外胚层基板内陷并闭合形成耳泡，耳泡随后经历复杂的形态发生过程形成膜迷路。Gata3缺陷小鼠胚胎的内耳形态发生受到严重影响，但表型的发生和基础尚不清楚。我们在这里表明，Gata3缺乏导致严重和独特的异常，在耳基板内陷。内陷的问题往往伴随着背侧和腹侧耳杯之间的形态边界的形成和背侧内淋巴特性的早熟外观。此外，在上皮闭合过程中，内淋巴结构域通常与耳上皮的其余部分分离。Gata3缺陷耳中几种细胞粘附介导基因的表达发生改变，表明Gata3控制早期耳上皮的粘附和形态发生运动。Gata3的失活还导致耳上皮和听神经节中Fgf10表达的丧失，表明Gata3是耳发育期间Fgf信号传导的重要调节剂。(c)2006爱思唯尔爱尔兰有限公司保留所有权利。
Inner ear develops from an induced surface ectoderm placode that invaginates and closes to form the otic vesicle, which then undergoes a complex morphogenetic process to form the membranous labyrinth. Inner ear morphogenesis is severely affected in Gata3 deficient mouse embryos, but the onset and basis of the phenotype has not been known. We show here that Gata3 deficiency leads to severe and unique abnormalities during otic placode invagination. The invagination problems are accompanied often by the formation of a morphological boundary between the dorsal and ventral otic cup and by the precocious appearance of dorsal endolymphatic characteristics. In addition, the endolymphatic domain often detaches from the rest of the otic epithelium during epithelial closure. The expression of several cell adhesion mediating genes is altered in Gata3 deficient ears suggesting that Gata3 controls adhesion and morphogenetic movements in early otic epithelium. Inactivation of Gata3 leads also to a loss of Fgf10 expression in otic epithelium and auditory ganglion demonstrating that Gata3 is an important regulator of Fgf-signalling during otic development. (c) 2006 Elsevier Ireland Ltd. All rights reserved.