DNA sequence variation and latitudinal associations in hsp23, hsp26 and hsp27 from natural populations of Drosophila melanogaster
DNA sequence variation and latitudinal associations in hsp23, hsp26 and hsp27 from natural populations of Drosophila melanogaster
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DOI：
10.1046/j.1365-294x.2002.01882.x
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发表时间：
2003-08-01
影响因子：
4.9
通讯作者：
Loeschcke, V
中科院分区：
文献类型：
--
作者：
Frydenberg, J;Hoffmann, AA;Loeschcke, V
热激基因被认为可能是环境胁迫抗性的候选基因。研究了来自荷兰和澳大利亚东部的果蝇小热休克基因(HSPs)hsp26和hsp27编码序列的核苷酸变异。在所有人群中，hsp26基因具有插入/缺失三个额外氨基酸和两个非同义改变的多态现象。Hsp27基因表现出两个非同义突变和三个同义突变。Hsp26基因的多态性表现为沿澳大利亚东海岸的纬向倾斜型。这一模式并没有被Ssps位于(3 L)P的反转中的事实所混淆，该反转也显示了澳大利亚东部的纬向倾斜。在上述hsp23基因变异中发现了相似的纬度变异，而hsp27等位基因频率不随纬度变化。这些发现表明，在自然种群中，两个shsps或紧密连锁的基因座上的变异正在进行选择。
Heat shock genes are considered to be likely candidate genes for environmental stress resistance. Nucleotide variation in the coding sequence of the small heat shock genes (hsps ) hsp26 and hsp27 from Drosophila melanogaster was studied in flies originating from the Netherlands and eastern Australia. The hsp26 gene was polymorphic for an insertion/deletion of three extra amino acids and two nonsynonymous changes in all populations. The hsp27 gene exhibited two nonsynonymous changes and three synonymous mutations. The hsp26 polymorphism showed a latitudinal cline along the east coast of Australia. This pattern was not confounded by the fact that the shsps are located in the inversion In(3 L)P which also shows a latitudinal cline in eastern Australia. A similar latitudinal cline was found for the previously described variation in hsp23 , while frequencies of hsp27 alleles did not change with latitude. These findings suggest that variation at two of the shsps or closely linked loci are under selection in natural populations of D. melanogaster.