Aptamer based voltammetric biosensor for Mycobacterium tuberculosis antigen ESAT-6 using a nanohybrid material composed of reduced graphene oxide and a metal-organic framework
Aptamer based voltammetric biosensor for Mycobacterium tuberculosis antigen ESAT-6 using a nanohybrid material composed of reduced graphene oxide and a metal-organic framework
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基于适体的伏安生物传感器，用于结核分枝杆菌抗原 ESAT-6，使用由还原氧化石墨烯和金属有机框架组成的纳米混合材料
DOI：
10.1007/s00604-018-2884-5
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发表时间：
2018-08-01
影响因子：
5.7
通讯作者：
Bai, Lijuan
中科院分区：
文献类型：
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作者：
Li, Linlin;Yuan, Yonghua;Bai, Lijuan
6 kDa早期分泌型抗原靶标ESAT-6是由结核分枝杆菌(MTB)分泌的一种毒力因子。本工作描述了一种用于ESAT-6超灵敏检测的伏安式适配传感器。在玻碳电极(GCE)上制备了掺杂金属有机骨架的还原氧化石墨烯(MOF-rGO)。这增加了电活性甲苯胺蓝(TB)的固定化，并促进了TB向修饰后的GCE的电子转移。利用铂/金核/壳(Pt@Au)纳米粒子在修饰电极上组装硫代化的ESAT-6结合适配子(EBA)，进一步放大对TB的响应。该方法的线性范围为1.0×10-4~2.0×10 2 ng⋅m L-1ESAT-6，检出限为3.3×10-5 ng⋅m L-1。在分析加标人血清时，该方法表现出令人满意的特异性和重复性。图解摘要用氧化石墨烯(RGO)和金属有机骨架(MOF)修饰的玻碳电极用于结核分枝杆菌抗原ESAT-6的伏安适配传感器的示意图。对ESAT-6的检出限为3.3×10-5 ng/mL。
The 6-kDa early secretory antigenic target referred to as ESAT-6 is a virulence factor secreted by Mycobacterium tuberculosis (MTB). This work describes a voltammetric aptasensor for ultrasensitive detection of ESAT-6. Reduced graphene oxide doped with metal-organic framework (MOF-rGO) was deposited on a glassy carbon electrode (GCE). This increases the immobilization of electroactive Toluidine Blue (TB) and facilitates the electron transfer from TB to the modified GCE. Platinum/gold core/shell (Pt@Au) nanoparticles were used to assemble thiolated ESAT-6 binding aptamer (EBA) on a modified electrode and to further amplify the response to TB. The modified GCE, typically operated at -0.36 V (vs. SCE), has a linear response in 1.0 × 10-4to 2.0 × 102ng⋅mL-1ESAT-6 concentration range, and the limit of detection (LOD) for ESAT-6 is as low as 3.3 × 10-5ng⋅mL-1. It exhibits satisfactory specificity and reproducibility when analyzing spiked human serum. Graphical abstract Schematic presentation of a voltammetric aptasensor for Mycobacterium tuberculosis antigen ESAT-6 using a glassy carbon electrode modified with reduced graphene oxide (rGO) and a metal-organic framework (MOF). The limit of detection for ESAT-6 is as low as 3.3 × 10-5ng/mL.