In Vivo Assessment of Thickness and Reflectivity in a Rat Outer Retinal Degeneration Model with Ultrahigh Resolution Optical Coherence Tomography
In Vivo Assessment of Thickness and Reflectivity in a Rat Outer Retinal Degeneration Model with Ultrahigh Resolution Optical Coherence Tomography
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DOI：
10.1167/iovs.11-8395
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发表时间：
2012-04-01
影响因子：
4.4
通讯作者：
Bizheva, Kostadinka
中科院分区：
文献类型：
--
作者：
Hariri, Sepideh;Moayed, Alireza A.;Bizheva, Kostadinka
目的.采用高分辨率光学相干断层扫描（UHR-OCT）技术，在体外定量评估碘酸钠诱导的大鼠视网膜外变性模型的视网膜损伤。通过尾静脉注射碘酸钠（40 mg/kg），在4只雌性Long Evans大鼠中诱导外部视网膜变性。使用半自动分割算法提取单个视网膜层的厚度和光学反射率的变化，并在体内使用UHR-OCT在注射后6小时，第1天，第3天和第7天以及长达3个月进行评估。苏木精和伊红（H&E）组织学用于确认UHR-OCT图像中观察到的形态学变化。UHR-OCT断层扫描显示，随着时间的推移，大鼠视网膜出现进行性结构损伤，例如肿胀、变薄、单个视网膜层完全崩解以及高反射细胞碎片聚集。在注射碘酸钠后6小时观察到感光器肿胀，随后是外层视网膜的进行性结构分解。注射后3个月，外层视网膜完全崩解，内核层（INL）与脉络膜直接接触。视网膜各层反射率随时间的变化与形态学变化相关。UHR-OCT允许在体外、非侵入性、纵向、定量评估碘酸钠啮齿动物模型中视网膜形态学和光学反射率的进行性变化。（Invest Ophthalmol维斯科学。2012;53：1982-1989）DOI：10.1167/iovs.11-8395
PURPOSE. To provide in vivo quantitative assessment of sodium iodate-induced retinal damage in a rat model of outer retinal degeneration using ultrahigh resolution optical coherence tomography (UHR-OCT).METHODS. Outer retinal degeneration was induced in four female Long Evans rats via tail vein injection of sodium iodate (40 mg/kg). Changes in the thickness and optical reflectivity of individual retinal layers were extracted using a semi-automatic segmentation algorithm and were assessed in vivo at 6 hours, days 1, 3, and 7, and up to 3 months post injection with UHR-OCT. Hematoxylin and eosin (H&E) histology was used to confirm the morphologic changes observed in the UHR-OCT images.RESULTS. UHR-OCT tomograms showed progressive structural damage in the rat retina over time, such as swelling, thinning, complete disintegration of individual retinal layers, and clustering of highly reflective cellular debris. Photoreceptor swelling was observed 6 hours after injection of sodium iodate, followed by progressive structural decomposition of the outer retina. At 3 months post injection, the outer retina was completely disintegrated, and the inner nuclear layer (INL) was in direct contact with the choroid. Changes in the reflectivity of individual retinal layers were observed over time and correlated well with the morphologic changes.CONCLUSIONS. UHR-OCT permits in vivo, noninvasive, longitudinal, quantitative assessment of the progressive changes in retinal morphology and optical reflectivity in a sodium iodate rodent model of outer retinal degeneration. (Invest Ophthalmol Vis Sci. 2012;53:1982-1989) DOI:10.1167/iovs.11-8395