7X multiplexed, optofluidic detection of nucleic acids for antibiotic-resistance bacterial screening
7X multiplexed, optofluidic detection of nucleic acids for antibiotic-resistance bacterial screening
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DOI：
10.1364/oe.402311
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发表时间：
2020-10-26
期刊：
影响因子：
3.8
通讯作者：
Schmidt, H.
中科院分区：
文献类型：
--
作者：
Meena, G. G.;Wall, T. A.;Schmidt, H.
快速准确地诊断对多种抗生素耐药的细菌感染需要开发新的生物传感器来区分检测多个靶标。这项工作证明了在光流体平台上进行耐药性细菌筛选的7倍多重检测。我们利用光谱多路复用多点激发来同时检测对应于细菌靶标和抗性基因的核酸链。这是通过集成在光流体设备中的多模干涉（MMI）波导实现的。我们采用了组合三色标记方案的核酸检测，以扩大其多重能力，以七种不同的核酸，代表三个物种和四个抗性基因。(C)根据OSA开放获取出版协议的条款，2020年美国光学学会
Rapid and accurate diagnosis of bacterial infections resistant to multiple antibiotics requires development of new bio-sensors for differentiated detection of multiple targets. This work demonstrates 7x multiplexed detection for antibiotic-resistance bacterial screening on an optofluidic platform. We utilize spectrally multiplexed multi-spot excitation fbr simultaneous detection of nucleic acid strands corresponding to bacterial targets and resistance genes. This is enabled by multi-mode interference (MMI) waveguides integrated in an optofluidic device. We employ a combinatorial three-color labeling scheme for the nucleic acid assays to scale up their multiplexing capability to seven different nucleic acids, representing three species and four resistance genes. (C) 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement