The Search Engine for Multi-Proteoform Complexes: An Online Tool for the Identification and Stoichiometry Determination of Protein Complexes.
The Search Engine for Multi-Proteoform Complexes: An Online Tool for the Identification and Stoichiometry Determination of Protein Complexes.
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DOI：
10.1002/cpbi.16
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发表时间：
2016-12-08
影响因子：
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通讯作者：
Kelleher, Neil L
中科院分区：
文献类型：
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作者：
Skinner, Owen S;Schachner, Luis F;Kelleher, Neil L
使用天然电喷雾的自上而下的质谱学的最新进展现在使以相对小的样本量以非目标模式分析完整的蛋白质复合体成为可能。在这里，我们描述了如何使用整个蛋白质组件的串联质谱学产生的输入数据来表征具有高分子专一性的同质和杂多复合体。所描述的工具是一个“多蛋白复合体搜索引擎”(SEMPC)，可以在线免费获得。结果是候选多蛋白形式复合体和评分指标的列表，它们被用来定义一个或多个独特的蛋白质亚基的不同集合，它们在完整复合体中的总体化学计量，以及它们翻译前和翻译后的修饰。因此，我们提出了一种从天然质谱学数据中识别和表征完整蛋白质复合体的方法。2016John Wiley&Sons，Inc.
Recent advances in top-down mass spectrometry using native electrospray now enable the analysis of intact protein complexes with relatively small sample amounts in an untargeted mode. Here, we describe how to characterize both homo- and heteropolymeric complexes with high molecular specificity using input data produced by tandem mass spectrometry of whole protein assemblies. The tool described is a "search engine for multi-proteoform complexes," (SEMPC) and is available for free online. The output is a list of candidate multi-proteoform complexes and scoring metrics, which are used to define a distinct set of one or more unique protein subunits, their overall stoichiometry in the intact complex, and their pre- and post-translational modifications. Thus, we present an approach for the identification and characterization of intact protein complexes from native mass spectrometry data. © 2016 by John Wiley & Sons, Inc.