Capillary electrophoretic examination of underivatized oligosaccharide mixtures released from immunoglobulin G antibodies and CTLA4Ig fusion protein.
Capillary electrophoretic examination of underivatized oligosaccharide mixtures released from immunoglobulin G antibodies and CTLA4Ig fusion protein.
复制标题
对免疫球蛋白 G 抗体和 CTLA4Ig 融合蛋白释放的未衍生化寡糖混合物进行毛细管电泳检查。
DOI：
10.1016/0021-9673(96)00458-x
复制
发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Karger,BL
中科院分区：
文献类型：
--
作者：
Greve,KF;Hughes,DE;Karger,BL
提出了植酸-硼酸缓冲液毛细管电泳分离低聚糖的方法。植酸离子配对剂的存在极大地提高了所研究的复杂混合物中低聚糖之间的分辨率，这一点通过分离来自各种免疫球蛋白G抗体和生物融合蛋白CTLA4Ig的低聚糖得到了证明。这些条件也可以溶解中性低聚糖，这通常是主要的CE限制。高性能阴离子交换色谱脉冲安培检测是低聚糖和糖分析的标准技术，作为参考方法分析一些复杂的低聚糖混合物。
A procedure is presented for the separation of underivatized oligosaccharides by capillary electrophoresis (CE) with a phytic acid-borate buffer system. The presence of the phytic acid ion-pairing agent greatly increases resolution between oligosaccharides in the complex mixtures studied, which was demonstrated by the separation of oligosaccharides originating from various immunoglobulin G antibodies and CTLA4Ig, a biologic fusion protein. The conditions also resolve neutral oligosaccharides, usually a major CE limitation. High-performance anion-exchange chromatography with pulsed amperometric detection, a standard technique for oligosaccharide and sugar analysis, is used as a reference method to analyze some of the complex oligosaccharide mixtures.
通过高性能毛细管电泳分析碳水化合物介导的异质性和糖蛋白 N 连接寡糖的表征
影响因子：
2.9
作者：
M. Taverna;A. Baillet;D. Biou;M. Schlüter;R. Werner;D. Ferrier
通讯作者：
D. Ferrier