Potential of mesenchymal stem cell sheets on periodontal regeneration: A systematic review of pre-clinical studies.
Potential of mesenchymal stem cell sheets on periodontal regeneration: A systematic review of pre-clinical studies.
复制标题
间充质干细胞片对牙周再生的潜力：临床前研究的系统评价。
DOI：
10.2174/1574888x17666220706092520
复制
发表时间：
2022
影响因子：
2.7
通讯作者：
M. Holzhausen
中科院分区：
文献类型：
--
作者：
A. Mendoza;Danilo Balzarini;Tomaz Alves;E. Rovai;M. Holzhausen
背景
利用间充质干细胞的细胞片层技术是牙周再生医学的一个高水平的策略。虽然最近的研究表明，MSCSs在增加牙齿支持组织和骨的作用，有没有一个系统的审查，特别是在原位动物模型中评估牙周再生。
目的
评价间充质干细胞片（MSCSs）在实验动物模型中与对照组相比对牙周再生的潜力。
方法
牙周缺损动物模型的临床前研究被视为合格。电子检索包括MEDLINE、Web of Science、EMBASE和LILACS数据库。根据系统性综述和荟萃分析声明指南的首选报告项目进行综述。
结果
从电子数据库检索获得的3989项研究中，共纳入17项。MSC包括牙囊MSC、牙周膜MSC、牙髓MSC、骨髓MSC、牙槽骨膜MSC和牙龈MSC。关于细胞片层诱导方案，大多数研究使用抗坏血酸（58.82%）。其他人使用的培养皿接枝有温度响应聚合物（41.18%）。在大多数研究中未发现不良反应。由于方法异质性，未考虑荟萃分析。PDL-MSCSs被证明是上级的牙周再生增强相比，对照组，但在诱导炎症微环境，DF-MSCSs更好。此外，与对照相比，DF-MSCS、DP-MSCS和BM-MSCS显示出改善的结果。
结论
MSCSs能促进牙周缺损动物模型的牙周组织再生。
BACKGROUND
Cell sheet technique using mesenchymal stem cells is a high-level strategy in periodontal regenerative medicine. Although recent studies have shown the role of MSCSs in increased dental supporting tissues and bone, there is not a systematic review focused specifically on assessing periodontal regeneration in orthotopic animal models.
OBJECTIVE
To evaluate the potential of mesenchymal stem cell sheets (MSCSs) on periodontal regeneration, compared to control, in experimental animal models.
METHODS
Pre-clinical studies in periodontal defects of animal models were considered eligible. The electronic search included the MEDLINE, Web of Science, EMBASE and LILACS databases. The review was conducted according to the Preferred Reporting Item for Systematic Reviews and Meta-Analyses statement guidelines.
RESULTS
A total of 17 of the 3989 studies obtained from the electronic database search were included. MSCSs included dental follicle (DF) MSCSs, periodontal ligament (PL) MSCSs, dental pulp (DP) MSCSs, bone marrow (BM) MSCSs, alveolar periosteal (AP) MSCSs and gingival (G) MSCSs. Regarding cell sheet inducing protocol, most of the studies used ascorbic acid (58.82%). Others used culture dishes grafted with a temperature-responsive polymer (41.18%). Adverse effects were not identified in the majority of studies. Meta-analysis was not considered because of methodological heterogeneities. PDL-MSCSs demonstrated to be superior for periodontal regeneration enhancement compared to control, but in an induced inflammatory microenvironment, DF-MSCSs were better. Moreover, DF-MSCSs, DP-MSCSs, and BM-MSCSs showed improved results compared to control.
CONCLUSION
MSCSs can improve periodontal regeneration in animal periodontal defect models.